Parallel pathways for rapid odor processing in lateral entorhinal cortex: Rate and temporal coding by layer 2 subcircuits
Parallel pathways for rapid odor processing in lateral entorhinal cortex: Rate and temporal coding by layer 2 subcircuits
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DOI：
10.1101/2021.08.19.456942
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发表时间：
2021-08
期刊：
影响因子：
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通讯作者：
S. Bitzenhofer;Elena A. Westeinde;Hanwen Zhang;J. Isaacson
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文献类型：
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作者：
S. Bitzenhofer;Elena A. Westeinde;Hanwen Zhang;J. Isaacson
嗅觉信息在外侧内嗅皮层 (LEC) 中由两类第 2 层 (L2) 主要神经元编码：扇形细胞和锥体细胞。然而，L2 神经元的功能特性尚不清楚。在这里，我们在清醒的小鼠中证明，L2 细胞在单次嗅觉过程中对气味做出快速反应，并且 LEC 对于辨别气味特性和强度至关重要。 L2 整体的群体分析表明，虽然速率编码可以区分气味特性，但放电速率与浓度的关系较弱，尖峰时间的变化代表气味强度。 L2 主细胞的传入嗅觉输入和与局部抑制电路的连接性不同，锥体细胞和扇形细胞尖峰的相对时间是气味强度编码的基础。下游，强度纯粹由海马 CA1 中的尖峰计时编码。总之，这些结果揭示了并行 LEC 子电路对气味信息的独特处理，并强调了高级嗅觉区域中时间编码的重要性。
Olfactory information is encoded in lateral entorhinal cortex (LEC) by two classes of layer 2 (L2) principal neurons: fan and pyramidal cells. However, the functional properties of L2 neurons are unclear. Here, we show in awake mice that L2 cells respond rapidly to odors during single sniffs and that LEC is essential for discrimination of odor identity and intensity. Population analyses of L2 ensembles reveals that while rate coding distinguishes odor identity, firing rates are weakly concentration-dependent and changes in spike timing represent odor intensity. L2 principal cells differ in afferent olfactory input and connectivity with local inhibitory circuits and the relative timing of pyramidal and fan cell spikes underlies odor intensity coding. Downstream, intensity is encoded purely by spike timing in hippocampal CA1. Together, these results reveal the unique processing of odor information by parallel LEC subcircuits and highlight the importance of temporal coding in higher olfactory areas.