Orexin exerts excitatory effects on reticulospinal neurons in the rat gigantocellular reticular nucleus through the activation of postsynaptic orexin-1 and orexin-2 receptors
Orexin exerts excitatory effects on reticulospinal neurons in the rat gigantocellular reticular nucleus through the activation of postsynaptic orexin-1 and orexin-2 receptors
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Orexin 通过激活突触后 orexin-1 和 orexin-2 受体对大鼠巨细胞网状核中的网状脊髓神经元产生兴奋作用
DOI：
10.1016/j.neulet.2017.05.048
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发表时间：
2017-07
影响因子：
2.5
通讯作者：
Zhang Jun
中科院分区：
文献类型：
--
作者：
Yang Nian;Wang Guan-Zhong;Wen Si-Yi;Qiao Qi-Cheng;Liu Yu-Hui;Zhang Jun
以往的研究表明，食欲素可能积极参与中枢运动控制。脑干网状核（Gi）是脑干运动抑制系统的一个重要组成部分。向下的食欲素能投射也到达Gi区，并且将食欲素微量注射到Gi中引起强烈的肌张力抑制。然而，食欲素对Gi神经元的调节作用尚不清楚。在本研究中，使用全细胞膜片钳记录，我们最初观察到食欲素以浓度依赖性方式在-70 mV的保持电位下引起Gi神经元的内向电流。通过结合电生理学和神经药理学方法，我们进一步确定食欲素诱导的内向电流是由突触后食欲素-1和食欲素-2受体的激活直接介导的。此外，食欲素不影响Gi神经元中的微型兴奋性和抑制性突触后电流的频率和幅度，这表明食欲素对这些神经元的神经传递没有影响。因此，本研究报道了食欲素对抑制性运动结构Gi的直接兴奋作用。这种调节可能被整合到食欲素在中枢运动控制中的作用中。
Previous studies have revealed that orexin may actively participate in central motor control. The gigantocellular reticular nucleus (Gi) is a key element of the brainstem motor inhibitory system. The descending orexinergic projections also reach Gi region, and microinjection of orexin into Gi causes robust muscle tone inhibition. However, the modulation effects of orexin on Gi neurons remain unclear. In the present study, using whole-cell patch-clamp recordings, we initially observed that orexin elicited an inward current in Gi neurons at a holding potential of −70 mV in a concentration-dependent manner. By combining electrophysiology with neuropharmacological methods, we further determined that the orexin-induced inward current was directly mediated by the activation of postsynaptic orexin-1 and orexin-2 receptors. Moreover, orexin did not affect the frequency and amplitude of miniature excitatory and inhibitory postsynaptic currents in Gi neurons, which suggests that orexin had no effects on neurotransmission to these neurons. Therefore, the direct excitatory effect of orexin on an inhibitory motor structure, the Gi, was reported in the present study. This modulation may be integrated into the role of orexin in central motor control.
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影响因子：
25
作者：
Robertson, Sabrina D.;Plummer, Nicholas W.;de Marchena, Jacqueline;Jensen, Patricia
通讯作者：
Jensen, Patricia
DOI：
10.1007/s12311-009-0146-0
发表时间：
2010-03
期刊：
The Cerebellum
影响因子：
--
作者：
Lei Yu;Xiao-Yang Zhang;Jun Zhang;Jing-Ning Zhu;Jian-jun Wang
通讯作者：
Lei Yu;Xiao-Yang Zhang;Jun Zhang;Jing-Ning Zhu;Jian-jun Wang
影响因子：
2.9
作者：
SIEGEL, JM;WHEELER, RL;MCGINTY, DJ
通讯作者：
MCGINTY, DJ
影响因子：
2.5
作者：
Jie Yan;Chao He;J. Xia;Dan Zhang;Zhian Hu
通讯作者：
Jie Yan;Chao He;J. Xia;Dan Zhang;Zhian Hu
影响因子：
2.9
作者：
Yoshifumi Kobayashi;K. Matsuyama;S. Mori
通讯作者：
Yoshifumi Kobayashi;K. Matsuyama;S. Mori