Reconstitution of CF1-depleted thylakoid membranes with complete and fragmented chloroplast ATPase. The role of the delta subunit for proton conduction through CF0.
Reconstitution of CF1-depleted thylakoid membranes with complete and fragmented chloroplast ATPase. The role of the delta subunit for proton conduction through CF0.
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用完整和片段的叶绿体 ATP 酶重建 CF1 耗尽的类囊体膜。
DOI：
10.1111/j.1432-1033.1986.tb10085.x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
W. Junge
中科院分区：
文献类型：
--
作者：
S. Engelbrecht;H. Lill;W. Junge
以菠菜、豌豆和玉米类囊体为材料，通过EDTA抽提和阴离子交换层析分离出叶绿体ATP酶（CF 1）。将CF 1纯化并通过HPLC拆分成完整的CF 1和缺乏δ和δ亚基的CF 1：CF 1（-δ）和CF 1（-δ）。用含酒精的缓冲液洗涤Mono-Q结合的CF 1，然后用无酒精洗脱，产生β亚基和单独的峰CF 1（-δ）和CF 1（-δ）。在表面活性剂存在下从Mono Q洗脱得到β δ片段，CF 1（-δ）和CF 1（-δ δ）。叶绿体CF 1耗尽EDTA提取。通过加入CF 1及其片段，在这些“EDTA囊泡”中获得了磷酸化的重构。CF 1、CF 1（-delta）和CF 1（-delta delta）在菠菜、豌豆和玉米之间具有交叉反应性。含有δ的CF 1总是比δ缺陷的CF 1重建更高的活性。β-δ片段和二环己基碳二亚胺（DCCD）抑制的CF 1也具有重建活性，而β和DCCD抑制的CF 1（-δ）则没有。这些结果支持了Junge，W.，Hong，Y.问：钱湖，加-地P.和Viale，A. [（1984）Proc. Natl Acad. Sci. USA 81，3078-3082]。
Chloroplast ATPase (CF1) was isolated from spinach, pea and maize thylakoids by EDTA extraction followed by anion-exchange chromatography. CF1 was purified and resolved by HPLC into integral CF1, and CF1 lacking the delta & epsilon subunits: CF1(-delta) and CF1(-epsilon). Washing Mono-Q-bound CF1 with alcohol-containing buffers followed by elution without alcohol produced the beta subunit and in separate peaks CF1(-delta) and CF1(-epsilon). Elution from Mono Q in the presence of tenside yielded a beta delta fragment, CF1(-delta) and CF1(-delta epsilon). Chloroplasts were CF1-depleted by EDTA extraction. Reconstitution of photophosphorylation in these 'EDTA vesicles' was obtained by addition of CF1 and its fragments. CF1, CF1(-delta) and CF1(-delta epsilon) were active with cross-reactivity between spinach, pea and maize. delta-containing CF1 always reconstituted higher activities than delta-deficient CF1. The beta delta fragment and dicyclohexylcarbodiimide (DCCD)-inhibited CF1 also were reconstitutively active while beta and DCCD-inhibited CF1(-delta) were not. These results support the notion that subunit delta can function as a stopcock to the CF0 proton channel as proposed by Junge, W., Hong, Y. Q., Qian, L. P. and Viale, A. [(1984) Proc. Natl Acad. Sci. USA 81, 3078-3082].
影响因子：
2.9
作者：
P. Karplus;K. Walsh;J. Herriott
通讯作者：
P. Karplus;K. Walsh;J. Herriott
制备重组活性形式的ε亚基和ε亚基缺陷型叶绿体耦合因子1。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Richter,ML;Patrie,WJ;McCarty,RE
通讯作者：
McCarty,RE
缺乏 delta 和 epsilon 亚基的耦合因子 1 与类囊体的特异性结合。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Patrie,WJ;McCarty,RE
通讯作者：
McCarty,RE