The interaction of macrophage and non-macrophage tropic isolates of HIV-1 with thymic and tonsillar dendritic cells in vitro
The interaction of macrophage and non-macrophage tropic isolates of HIV-1 with thymic and tonsillar dendritic cells in vitro
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DOI：
10.1084/jem.183.4.1851
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发表时间：
1996-04-01
影响因子：
15.3
通讯作者：
Shortman, K
中科院分区：
文献类型：
--
作者：
Cameron, PU;Lowe, MG;Shortman, K
测试从胸腺和扁桃体分离的树突状细胞对嗜性于巨噬细胞或T细胞系的HIV-1株的易感性。通过细胞分选纯化DC，并且在感染前表达高水平的CD 4和HLA-DR，并且缺乏T、B、NK细胞或巨噬细胞的标记。用能感染巨噬细胞的HIV-1毒株脉冲后，发现病毒进入并逆转录。在第一个36小时期间，DC和巨噬细胞中gag序列的PCR信号增加。相反，在用能够感染T细胞百合的HIV-1脉冲巨噬细胞或DC后，几乎没有发现任何病毒DNA。HIV-1致敏的DC能够在同种异体或超抗原反应中将感染传递给反应性T细胞，选择能够感染淋巴样DC和其他表达CD 4的DC并在随后的免疫反应中将其转移到T细胞的病毒可能为观察到的嗜巨噬细胞的HIV-1在体内传播后占优势提供了一种机制。
Dendritic cells isolated from thymus and tonsil were tested for susceptibility to HIV-1 strains that are tropic for macrophages or for T cell lines. DCs were purified by cell sorting and before infection expressed high levels of CD4 and HLA-DR and lacked markers for T, B, NK cells, or macrophages. Viral entry and revel-se transcription was found after pulsing with strains of HIV-1 that could infect macrophages. During the first 36 h the PCR signals for gag sequences increased in DCs and macrophages. In contrast little if any viral DNA was found after pulsing macrophages or DCs with HIV-1 that was able to infect T cell lilies. DCs pulsed with HIV-1 were able to transmit infection to responding T cells during an allogeneic or superantigen response.Selection for virus able to infect lymphoid DCs and other DCs expressing CD4 and its transfer to T cells during subsequent immune responses may provide a mechanism for the observed predominance of macrophage-tropic HIV-1 after in vivo transmission.