Rational, yet simple, design and synthesis of an antifreeze-protein inspired polymer for cellular cryopreservation.
Rational, yet simple, design and synthesis of an antifreeze-protein inspired polymer for cellular cryopreservation.
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DOI：
10.1039/c5cc04647e
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发表时间：
2015-08-21
期刊：
影响因子：
--
通讯作者：
Gibson MI
中科院分区：
文献类型：
--
作者：
Mitchell DE;Cameron NR;Gibson MI
设计并合成了一种基于交替带电侧链的结构简单的合成聚合物，以模拟抗冻蛋白。发现该聚合物可增强红细胞的冷冻保存。抗冻（糖）蛋白AF（G）Ps是有效的冰重结晶抑制剂，其是增强供体组织/细胞的冷冻保存的期望性质。在这里，我们提出了一种新的，仿生的，冰重结晶抑制聚合物来自廉价的商品聚合物，基于两性电解质结构的合理合成。该聚合物用于增强红细胞的冷冻保存，证明了组织储存的大分子溶液。
A structurally simple synthetic polymer based on alternating charged side chains is designed and synthesised to mimic antifreeze proteins. The polymer is found to enhance the cryopreservation of red blood cells. Antifreeze (glyco) proteins AF(G)Ps are potent ice recrystallization inhibitors, which is a desirable property to enhance cryopreservation of donor tissue/cells. Here we present the rational synthesis of a new, biomimetic, ice-recrystallization inhibiting polymer derived from a cheap commodity polymer, based on an ampholyte structure. The polymer is used to enhance the cryopreservation of red blood cells, demonstrating a macromolecular solution to tissue storage.