COMPARISON OF WESTERN BLOT (IMMUNOBLOT) AND GLYCOPROTEIN-G-SPECIFIC IMMUNODOT ENZYME ASSAY FOR DETECTING ANTIBODIES TO HERPES-SIMPLEX VIRUS TYPE-1 AND TYPE-2 IN HUMAN-SERA
COMPARISON OF WESTERN BLOT (IMMUNOBLOT) AND GLYCOPROTEIN-G-SPECIFIC IMMUNODOT ENZYME ASSAY FOR DETECTING ANTIBODIES TO HERPES-SIMPLEX VIRUS TYPE-1 AND TYPE-2 IN HUMAN-SERA
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DOI：
10.1128/jcm.26.4.662-667.1988
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发表时间：
1988-04-01
影响因子：
9.4
通讯作者：
COREY, L
中科院分区：
文献类型：
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作者：
ASHLEY, RL;MILITONI, J;COREY, L
用免疫印迹技术(WBA)和免疫斑点酶试验(IEAS)检测生殖器疱疹患者血清中单纯疱疹病毒1型(HSV-1)和单纯疱疹病毒2型(HSV-2)特异性抗体。在检测的137份血清样本中，没有一份是WBA或IEA的错别型。两种试验对初发HSV-2感染后至少21天的血清或HSV-2生殖器复发期间采集的血清最敏感：76份血清样本中有75份(99%)WBA检测HSV-2抗体阳性，IEA检测73份(96%)阳性。在初发生殖器HSV-2发病21天前的血清中，WBA和IEA分别检出25%和8%的HSV-2抗体。在培养证实的初级生殖器HSV-1感染患者中，WBA在初级生殖器HSV-1感染发生至少21天后提取的17份血清样本中有16份(94%)检测到HSV-1蛋白抗体，而IEA检测的17份血清样本中有9份(53%)检测出GG-1。WBA和IEA都是首次发病后康复或有症状或无症状复发性生殖器疱疹患者的HSV-2抗体准确而敏感的检测方法。在检测原发HSV-1生殖器疱疹后的血清转换方面，WBA比IEA更敏感，尽管这两种方法都可能漏掉早期发生HSV-2血清转换的人。
Sera from patients with culture-proven genital herpes infections were tested for herpes simplex virus type 1 (HSV-1)- and HSV-2-specific antibodies by both a Western blot (immunoblot) technique (WBA) and immunodot enzyme assays (IEAs) specific for HSV-1 or HSV-2 glycoprotein G (gG). Of 137 serum samples tested, none was mistyped by either WBA or IEA. Both tests were most sensitive with sera obtained at least 21 days after onset of primary HSV-2 infections or sera drawn during recurrent HSV-2 genital episodes: 75 of 76 (99%) such serum samples were positive for HSV-2 antibody by WBA and 73 of 76 (96%) were positive by IEA. Of sera drawn earlier than 21 days from onset of primary genital HSV-2, antibodies to HSV-2 were detected in 25% by WBA and 8% by IEA. In patients with culture-proven primary genital HSV-1 infection, WBA detected antibodies to HSV-1 proteins in 16 of 17 (94%) serum samples drawn at least 21 days after onset of primary genital HSV-1 infection, compared with 9 of 17 (53%) serum samples tested for gG-1 by IEA. Both WBA and IEA are accurate and sensitive tests for HSV-2 antibody in patients convalescing from a first episode or having symptomatic or asymptomatic recurrent genital herpes. WBA was more sensitive than IEA in detecting seroconversion following primary HSV-1 genital herpes, although both assays may miss persons undergoing early seroconversion to HSV-2.