Runx1t1 Regulates the Neuronal Differentiation of Radial Glial Cells From the Rat Hippocampus
Runx1t1 Regulates the Neuronal Differentiation of Radial Glial Cells From the Rat Hippocampus
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DOI：
10.5966/sctm.2014-0158
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发表时间：
2015-01
影响因子：
6
通讯作者：
Zou Linqing;Jin Guohua;Liu Haoming;Tao Xuelei;Qin Jianbing;T. Mei-ling
中科院分区：
文献类型：
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作者：
Zou Linqing;Jin Guohua;Liu Haoming;Tao Xuelei;Qin Jianbing;T. Mei-ling
相对于其他器官的水平，大脑的 Runx1t1 水平最高。 Runx1t1 可能在神经系统的分化/发育中具有作为转录辅阻遏物的调节功能。神经发生需要调节祖细胞增殖并激活神经元分化过程的因子。然而，Runx1t1 在海马神经发生中的确切作用尚不清楚。我们使用慢病毒载体通过 Runx1t1-RNA 干扰敲低海马放射状胶质细胞 (RGC) 中的 Runx1t1。我们还使用 LV-Runx1t1 在体外诱导 Runx1t1 过度表达。我们提供的实验证据表明，Runx1t1 表达减少会减少 RGC 的神经元分化，而 Runx1t1 表达增加会导致更多数量的 RGC 分化为神经元。我们得出结论，Runx1t1 可能参与 RGC 分化为神经元的过程。
The brain has the highest Runx1t1 level relative to the levels in other organs. Runx1t1 might have a regulatory function as a transcriptional corepressor in the differentiation/development of the nervous system. Neurogenesis requires factors that regulate the proliferation of progenitors and activate the neuronal differentiation process. However, the precise role of Runx1t1 in hippocampal neurogenesis is unclear. We knocked down Runx1t1 in hippocampal radial glial cells (RGCs) with Runx1t1‐RNA interference using lentiviral vectors. We also used LV‐Runx1t1 to induce Runx1t1 overexpression in vitro. We have provided experimental evidence that decreased Runx1t1 expression reduced the neuronal differentiation of RGCs, and increased Runx1t1 expression caused a greater number of RGCs to differentiate into neurons. We have concluded that Runx1t1 could be involved in the process through which RGCs differentiate into neurons.