PH directly regulates epidermal permeability barrier homeostasis, and stratum corneum integrity/cohesion
PH directly regulates epidermal permeability barrier homeostasis, and stratum corneum integrity/cohesion
复制标题
DOI：
10.1046/j.1523-1747.2003.12365.x
复制
发表时间：
2003-08-01
影响因子：
6.5
通讯作者：
Elias, PM
中科院分区：
文献类型：
--
作者：
Hachem, JP;Crumrine, D;Elias, PM
角质层暴露于中性pH缓冲液和阻断酸化机制都会扰乱皮肤渗透性屏障的稳态和角质层的完整性/凝聚力，但这些方法都引入了潜在的混杂变量。为了研究独立于水合作用的角质层中和的后果，我们将丙二醇：乙醇（7:3）中的1,1,3,3-四甲基胍或1,8-重氮双环[5,4,0]11 -7-烯两种化学上不相关的超碱应用于无毛小鼠皮肤，并评估单独的pH变化是否调节皮肤渗透性屏障功能和角质层完整性/凝聚力，以及相关机制。1,1,3,3-四甲基胍和1,8-重氮双环[5,4,0]十一-7-烯的应用都增加了皮肤表面pH值，同时导致屏障稳态和角质层完整性/内聚性异常。后者可归因于快速激活(
Both exposure of stratum corneum to neutral pH buffers and blockade of acidification mechanisms disturb cutaneous permeability barrier homeostasis and stratum corneum integrity/cohesion, but these approaches all introduce potentially confounding variables. To study the consequences of stratum corneum neutralization, independent of hydration, we applied two chemically unrelated superbases, 1,1,3,3-tetramethylguanidine or 1,8-diazabicyclo [5,4,0] undec-7-ene, in propylene glycol:ethanol (7:3) to hairless mouse skin and assessed whether discrete pH changes alone regulate cutaneous permeability barrier function and stratum corneum integrity/cohesion, as well as the responsible mechanisms. Both 1,1,3,3-tetramethylguanidine and 1,8-diazabicyclo [5,4,0] undec-7-ene applications increased skin surface pH in parallel with abnormalities in both barrier homeostasis and stratum corneum integrity/cohesion. The latter was attributable to rapid activation (