Functional characterization of MIMP for its adhesion to the intestinal epithelium
Functional characterization of MIMP for its adhesion to the intestinal epithelium
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MIMP 与肠上皮粘附的功能表征
DOI：
10.2741/3842
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发表时间：
2011-06-01
影响因子：
3.1
通讯作者：
Qin, Huanlong
中科院分区：
文献类型：
--
作者：
Liu, Zhihua;Shen, Tongyi;Qin, Huanlong
植物乳杆菌CGMCC 1258整合膜蛋白中的微整合膜蛋白(MIMP)能与粘蛋白结合，并能拮抗侵袭性和致病性大肠杆菌的粘附性。为了进一步研究MIMP的功能，我们在体内外研究了MIMP对肠道通透性、紧密连接(TJ)蛋白表达和TJ超微结构的影响。我们还测定了MIMP与树突状细胞(DC)之间的相互作用。我们观察到，MIMP降低了NCM460细胞单层和IL-10(-/-)小鼠的肠道通透性，恢复了TJ蛋白的表达和分布。MIMP通过与DC-SIGN结合，与未成熟(I)DC黏附，诱导DC产生抗炎细胞因子并介导Th2分化。此外，MIMP还能刺激IL-10(-/-)小鼠结肠黏膜中抗炎细胞因子的表达，减轻结肠炎。综上所述，MIMP是植物乳杆菌发挥保护作用的主要功能成分，可能是一种潜在的肠道疾病治疗剂。
The micro integral membrane protein (MIMP), the domain within the integral membrane protein of Lactobacillus plantarum CGMCC 1258, has been shown to adhere to mucin and antagonize the adhesion of enteroinvasive E. coli and enteropathogenic E. coli. To further characterize the functions of MIMP, we investigated its effects on the intestinal permeability, expression of tight junction (TJ) proteins and TJ ultrastructure in vitro and in vivo. We also determined the interaction between MIMP and dendritic cells (DCs). We observed that MIMP reduced intestinal permeability and restored the expression and distribution of TJ proteins in both NCM460 cell monolayers and in IL-10(-/-) mice. MIMP adhered to immature (i) DCs by binding to DC-SIGN, and induced DCs to produce anti-inflammatory cytokines and to mediate Th2 differentiation. Moreover, MIMP stimulated the expression of anti-inflammatory cytokines in colonic mucosa and attenuated colitis in IL-10(-/-) mice. In conclusion, MIMP is the main functional component of L. plantarum that contributes to its protective effects, and thus may be a potential therapeutic agent for intestinal diseases.