Splenic lymph follicles generate immunoglobulin M-producing B cells in primary biliary cirrhosis.
Splenic lymph follicles generate immunoglobulin M-producing B cells in primary biliary cirrhosis.
复制标题
在原发性胆汁性肝硬化中，脾淋巴滤泡产生产生免疫球蛋白 M 的 B 细胞。
DOI：
10.1111/hepr.12231
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Miyakawa H.
中科院分区：
文献类型：
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作者：
Kikuchi K;Tsuneyama K;Yamada H;Kajiyama Y;Matsumoto K;Tsunashima H;Yamashita R;Takai A;Negishi M;Hara M;Moritoki Y;Miyakawa H.
目的对原发性胆汁性肝硬化（PBC）患者的脾脏进行免疫组织化学分析，揭示免疫球蛋白（Ig）M在原发性胆汁性肝硬化（PBC）中的产生部位。方法在肝衰竭患者尸检时采集脾组织标本。用脾组织标本进行IgM、CD21和CXCL13免疫染色。结果8例PBC患者中有5例在CD21阳性淋巴滤泡中有IgM阳性细胞聚集，8例慢性丙型肝炎病毒感染患者中无IgM阳性细胞聚集。CXCL13阳性细胞也聚集在IgM阳性细胞聚集的淋巴滤泡中心。结论PBC的脾脏产生过量的IgM。此外，研究表明CXCL13阳性的滤泡树突状细胞可能参与了这一过程。
AimTo reveal the site of immunoglobulin (Ig)M production in primary biliary cirrhosis (PBC) we performed immunohistochemical analysis on spleens collected from patients with PBC.MethodsSplenic tissue samples were collected at the time of the autopsy from patients with hepatic failure. Immunostaining for IgM, CD21 and CXCL13 were performed using the splenic tissue samples.ResultsThe samples from five out of eight cases with PBC but not in eight cases of chronic hepatitis C virus infection showed accumulation of IgM positive cells in CD21 positive lymph follicles. The CXCL13 positive cells also accumulated in the center of the lymph follicles where the IgM positive cells accumulated.ConclusionThe present results suggest that excess IgM is produced from the spleen of PBC. Furthermore, it was suggested that CXCL13 positive follicular dendritic cells possibly contribute to this process.