Genome sequencing and pathogenicity analysis of Senecavirus A, SVA-FJLY.
Genome sequencing and pathogenicity analysis of Senecavirus A, SVA-FJLY.
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DOI：
10.11843/j.issn.0366-6964.2020.04.018
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发表时间：
2020-01-01
期刊：
影响因子：
--
通讯作者：
Xu, L.
中科院分区：
文献类型：
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作者：
Lai, Long-yong;Xiaolong, Liu;Xu, L.
本研究的目的是阐明Senecavirus A（FJLY株）的基因组特征、同源性和致病性。以从福建省某养殖场分离的SVA-FJLY菌株为研究对象。根据SVV-001全基因组序列（GenBank No.DQ641257.1）设计5对引物，通过RT-PCR、测序和拼接等方法获得SVA-FJLY全基因组序列。3-4周龄的仔猪也通过鼻内喷雾感染该菌株。结果表明，SVA-FJLY菌株基因组全长为7 275 bp（不含PolyA）。它编码一种多聚蛋白、VP 1蛋白和VP 2蛋白。SVA-FJLY株与参考株、HeB 01 -2017株（MF967574.1）同源性较高，最高同源性达98.5%，与SVA原型株SVV-001（DQ 641257.1）同源性较低，达93.9%。SVA-FJLY株与国内分离的多株Senecavirus A属同一分支，国内与HeB 01 -2017株亲缘关系最近，国外与Colombia-2016株（KX857728.1）亲缘关系最近。动物实验表明，实验组的三只动物中有两只患有这种疾病，而对照组的三只动物都保持正常。试验组攻毒后10天6头仔猪全部存活。通过基因组测序获得了SVA-FJLY菌株的全基因组序列。阐明了SVA-FJLY株的基因组特征及其与其它菌株的同源性。同时还揭示了SVA-FJLY菌株的致病性。为SVA的反向遗传学研究和疫苗研制提供了参考，并丰富了SVA基因组信息数据库。
The aim of this study was to clarify genomic characteristics, homology, and pathogenicity of Senecavirus A (strain FJLY). Strain SVA-FJLY isolated from a farm in Fujian Province was used as focus of study. Five pairs of primers were designed based on whole-genome sequence of SVV-001 (GenBank No. DQ641257.1) and whole-genome sequence of strain SVA-FJLY was obtained and analyzed by RT-PCR, sequencing, and splicing. Piglets aged 3-4 weeks old were also infected with this strain by intranasal spray. Results showed that genome of strain SVA-FJLY had a full length of 7 275 bp (excluding PolyA). It encoded a polyprotein, VP1 protein, and VP2 protein. Strain SVA-FJLY had high homology with reference strain and HeB01-2017 strain (MF967574.1), with highest homology reaching 98.5%, while it had low homology with SVA prototype strain SVV-001 (DQ641257.1), reaching 93.9%. Strain SVA-FJLY belonged to the same branch as several Senecavirus A strains isolated in China, and domestically had closest relationship with strain HeB01-2017, while regarding foreign strains it had closest relationship with Colombia-2016 (KX857728.1). Animal experiments showed that two of three animals in experimental group suffered from the disease, while all three animals in control group remained normal. All six piglets survived 10 days after challenge of experimental group. Whole-genome sequence of strain SVA-FJLY was obtained by genome sequencing. Genomic characteristics of strain SVA-FJLY and its homology with other strains were clarified. Pathogenicity of strain SVA-FJLY was also revealed. Moreover, a reference for reverse genetics and vaccine development for SVA was provided, and database with genome information on SVA was also enriched.