MiR-145-5p alleviates hypoxia/reoxygenation-induced cardiac microvascular endothelial cell injury in coronary heart disease by inhibiting Smad4 expression
MiR-145-5p alleviates hypoxia/reoxygenation-induced cardiac microvascular endothelial cell injury in coronary heart disease by inhibiting Smad4 expression
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DOI：
10.26355/eurrev_202005_21192
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发表时间：
2020-05-01
影响因子：
3.3
通讯作者：
Xue, A-G
中科院分区：
文献类型：
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作者：
Li, L-L;Mao, C-D;Xue, A-G
目的：探讨miR-145-5p对冠心病（CHD）缺氧/复氧（H/R）诱导的心脏微血管内皮细胞（CMEC）损伤的影响及机制。 患者与方法：纳入冠心病患者（n=96）和健康志愿者（n=96），建立CMECs H/R损伤模型。通过逆转录聚合酶链式反应 (RT-PCR) 定量细胞中 miR-145-5p 和母亲针对十五麻痹同源物 4 (Smad4) mRNA 的表达。然后，将miR-145-5p模拟物和Smad4抑制剂转染至CMEC中。采用细胞计数试剂盒8（CCK-8）进行增殖检测，流式细胞仪进行凋亡检测，Western Blot检测凋亡相关蛋白和Smad4蛋白的表达量。结果：冠心病患者血清中miR-1455p的表达量显着低于健康个体。 miR-145-5p诊断CHD的曲线下面积（AUC）为0.894，miR-145-5p的表达与Smad4的表达呈负相关（p
OBJECTIVE: To investigate the effects and mechanism of miR-145-5p on hypoxia/reoxygenation (H/R)-induced cardiac microvascular endothelial cell (CMEC) injury in coronary heart disease (CHD).PATIENTS AND METHODS: Patients with CHD (n=96) and healthy volunteers (n= 96) were enrolled, and H/R injury model of CMECs was established. The expression of miR-145-5p and mothers against decapentaplegic homolog 4 (Smad4) mRNA in cells was quantified with reverse transcription polymerase chain reaction (RT-PCR). Then, miR-145-5p mimics and Smad4 inhibitor were transfected into CMECs. Cell counting kit-8 (CCK-8) was employed for proliferation detection, flow cytometry for apoptosis detection, and Western Blot for measuring the expression of apoptosis-related proteins and Smad4 protein.RESULTS: The expression of serum miR-1455p in patients with CHD was significantly lower than that in healthy individuals. The area under the curve (AUC) of miR-145-5p in diagnosing CHD was 0.894, and the expression of miR-145-5p was negatively correlated with that of Smad4 (p