Assessment of SENP3-interacting proteins in hepatocytes treated with diethylnitrosamine by BioID assay.
Assessment of SENP3-interacting proteins in hepatocytes treated with diethylnitrosamine by BioID assay.
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通过 BioID 测定评估经二乙基亚硝胺处理的肝细胞中 SENP3 相互作用蛋白
DOI：
10.1093/abbs/gmab096
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发表时间：
2021-08-31
影响因子：
3.7
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Chen F;Yan H;Guo C;Zhu H;Yi J;Sun X;Yang J
蛋白质的SUMO化调节细胞行为，并被小泛素样修饰物（SUMO）特异性蛋白酶（SENP）可逆地去除。SENP家族成员SENP 3参与SUMO 2/3去缀合，并且已经报道了感知细胞应激并在几种人类癌细胞和巨噬细胞中积累。我们以前报道过，Senp 3基因敲除的杂合子小鼠显示出较小的肝脏，但SENP 3和SUMO化底物的相关机制仍不清楚。因此，在这项研究中，我们研究了与SENP 3相互作用的蛋白质，以及用异生质二乙基亚硝胺（DEN）处理的肝细胞中的变化，DEN在肝脏中特异性转化并诱导DNA双链断裂。我们的数据显示，一定量的SENP 3存在于正常的、未处理的肝细胞中;然而，DEN处理促进了SENP 3的快速积累。SENP 3主要定位于细胞核，DEN处理2 h后，SENP 3在细胞质中的表达明显增加。最近的邻近依赖性生物素化（BioID）方法的应用导致了310个SENP 3相互作用蛋白的鉴定，这些蛋白不仅参与基因转录，而且参与RNA剪接、蛋白质折叠和代谢。此外，在DEN暴露短时间后，核糖体蛋白以及与线粒体ATP合成、膜转运和胆汁酸合成相关的蛋白，而不是DNA修复蛋白，被鉴定。这项研究为SENP 3的多种调节作用提供了见解，BioID方法似乎可以有效地识别生理相关的不溶性蛋白质。
SUMOylation of proteins regulates cell behaviors and is reversibly removed by small ubiquitin-like modifier (SUMO)-specific proteases (SENPs). The SENP family member SENP3 is involved in SUMO2/3 deconjugation and has been reported to sense cell stress and accumulate in several human cancer cells and macrophages. We previously reported that Senp3-knockout heterozygous mice showed smaller liver, but the pertinent mechanisms of SENP3 and SUMOylated substrates remain unclear. Thus, in this study, we investigated the interacting proteins with SENP3 and the alteration in hepatocytes treated with the xenobiotic diethylnitrosamine (DEN), which is specifically transformed in the liver and induces DNA double-strand breaks. Our data revealed that a certain amount of SENP3 was present in normal, untreated hepatocytes; however, DEN treatment promoted rapid SENP3 accumulation. SENP3 was mainly localized in the nuclei, and its level was significantly increased in the cytoplasm after 2 h of DEN treatment. The application of the recent proximity-dependent biotinylation (BioID) method led to the identification of 310 SENP3-interacting proteins that were involved in not only gene transcription but also RNA splicing, protein folding, and metabolism. Furthermore, after DEN exposure for a short duration, ribosomal proteins as well as proteins associated with mitochondrial ATP synthesis, membrane transport, and bile acid synthesis, rather than DNA repair proteins, were identified. This study provides insights into the diverse regulatory roles of SENP3, and the BioID method seems to be efficient for identifying physiologically relevant insoluble proteins.
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影响因子：
16.6
作者：
Lumpkin RJ;Gu H;Zhu Y;Leonard M;Ahmad AS;Clauser KR;Meyer JG;Bennett EJ;Komives EA
通讯作者：
Komives EA
影响因子：
3.3
作者：
Castle CD;Cassimere EK;Denicourt C
通讯作者：
Denicourt C
SENP3 通过拮抗 SUMO2/3 靶向泛素化和降解来调节整体蛋白质周转和 Sp1 水平
影响因子：
21.1
作者：
Wang, Ming;Sang, Jing;Yi, Jing
通讯作者：
Yi, Jing
影响因子：
16.6
作者：
Lamoliatte, Frederic;Caron, Danielle;Thibault, Pierre
通讯作者：
Thibault, Pierre
DOI：
10.1007/978-1-62703-311-4_11
发表时间：
2013-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Roszik, Janos;Toth, Gabor;Vereb, Gyorgy
通讯作者：
Vereb, Gyorgy