Mapping the electrophysiological and morphological properties of CA1 pyramidal neurons along the longitudinal hippocampal axis.
Mapping the electrophysiological and morphological properties of CA1 pyramidal neurons along the longitudinal hippocampal axis.
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DOI：
10.1002/hipo.22526
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发表时间：
2016-03
期刊：
影响因子：
3.5
通讯作者：
Johnston D
中科院分区：
文献类型：
--
作者：
Malik R;Dougherty KA;Parikh K;Byrne C;Johnston D
海马背侧、中间和腹侧区域的行为角色、解剖连接和基因表达模式的差异得到了很好的表征。然而，相对较少的研究集中在比较位于海马不同背腹侧范围的神经元的生理特性。最近，我们报道背侧CA 1神经元比腹侧神经元更不容易兴奋。关于中间海马体中的神经元如何与背侧和腹侧端的神经元进行比较的信息很少或根本没有。此外，我们也不知道沿着背腹轴的性质转变是逐渐的还是分段的。在这项研究中，我们开发了一个统计模型来预测横向海马脑片的背腹侧位置。使用电流钳记录结合这个模型，我们发现，在背侧，中间和腹侧海马CA 1神经元有不同的电生理和形态学特性和过渡，在大多数（但不是全部）这些属性从腹侧到背侧端是渐进的。通过线性和分段回归分析，我们发现输入电阻和静息膜电位沿V-D轴呈沿着线性变化。有趣的是，共振频率、反弹斜率、放射层树突分支和动作电位特性的转变沿V-D轴沿着分段。总之，这项研究的结果突出了沿着整个海马纵轴的CA 1神经元特性的异质性。
Differences in behavioral roles, anatomical connectivity and gene expression patterns in the dorsal, intermediate and ventral regions of the hippocampus are well characterized. Relatively fewer studies have, however, focused on comparing the physiological properties of neurons located at different dorsoventral extents of the hippocampus. Recently we reported that dorsal CA1 neurons are less excitable than ventral neurons. There is little or no information for how neurons in the intermediate hippocampus compare to those from the dorsal and ventral ends. Also, it is not known whether the transition of properties along the dorsoventral axis is gradual or segmented. In this study, we developed a statistical model to predict the dorsoventral position of transverse hippocampal slices. Using current clamp recordings combined with this model, we found that CA1 neurons in dorsal, intermediate and ventral hippocampus have distinct electrophysiological and morphological properties and that the transition in most (but not all) of these properties from the ventral to dorsal end is gradual. Using linear and segmented regression analyses, we found that input resistance and resting membrane potential changed linearly along the V–D axis. Interestingly, the transition in resonance frequency, rebound slope, dendritic branching in stratum radiatum and action potential properties was segmented along the V–D axis. Together, the findings from this study highlight the heterogeneity in CA1 neuronal properties along the entire longitudinal axis of hippocampus.