Electrochromatographic performance of conventional and polar-embedded C16 silica monolithic stationary phases.
Electrochromatographic performance of conventional and polar-embedded C16 silica monolithic stationary phases.
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DOI：
10.1002/jssc.201000439
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发表时间：
2010-11
影响因子：
3.1
通讯作者：
Fanggui Ye;Baojun Huang;Shun Wang;Shulin Zhao
中科院分区：
文献类型：
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作者：
Fanggui Ye;Baojun Huang;Shun Wang;Shulin Zhao
描述了一种新型的整体硅胶柱，该柱具有极性嵌入的酰胺-仲胺基团，与C16官能团连接，用于RP-CEC。以3-（2-氨乙基氨基）丙基三甲氧基硅烷和十六酰氯为原料合成了酰胺-仲胺烷氧基硅烷。然后将硅烷化试剂键合到硅胶整体柱基质上，制得十六酰胺-仲胺键合硅胶（HDAIS）整体柱。研究了HDAIS整体柱对中性、碱性和极性溶质的电色谱分离性能，并与十六烷基键合硅胶整体柱进行了比较。HDAIS整体柱显示减少的疏水保留特性，在5个疏水性的正烷基苯和4个极性酚的分离相比，十六烷基键合硅胶整体柱。以含30%v/v乙腈的10 mM硼酸盐缓冲液（pH7.5）为移动的，HDAIS整体柱分离四种芳香胺、阿替洛尔和美托洛尔的试验碱性混合物时，观察到硅醇活性大大降低。比较结果表明，极性和碱性混合物在HDAIS整体柱上的RP-CEC的良好性能，也显示了进一步的应用前景。
A novel monolithic silica column that has a polar-embedded amide-secondary amine group linking with C16 functionality for RP-CEC is described. The amide-secondary aminealkyloxysilane was synthesized by the reaction of 3-(2-aminoethylamino) propyltrimethoxysilane with hexadecanoyl chloride. Then, the silylant agent was bonded to the silica monolith matrix to produce hexadecanamide-secondary amine bonded silica (HDAIS) monolithic column. The electrochromatographic performance of HDAIS monolithic column for the separation of neutral, basic and polar solutes was studied, which was compared to that using the hexadecyl bonded silica monolithic column. The HDAIS monolithic column displayed reduced hydrophobic retention characteristics in the separation of five hydrophobic n-alkylbenzenes and four polar phenols when compared to the hexadecyl bonded silica monolithic column. A very much reduced silanol activity of HDAIS monolithic column was observed in the separation of test basic mixture including four aromatic amines, atenolol and metoprolol with 10 mM borate buffer (pH 7.5) containing 30% v/v ACN as the mobile phase. The comparison results indicate good performance for both polar and basic mixtures on HDAIS monolithic column in RP-CEC, and also show promising results for further applications.