BETA-KETOACYL-ACP SYNTHASE-I OF ESCHERICHIA-COLI - NUCLEOTIDE-SEQUENCE OF THE FABB GENE AND IDENTIFICATION OF THE CERULENIN BINDING RESIDUE
BETA-KETOACYL-ACP SYNTHASE-I OF ESCHERICHIA-COLI - NUCLEOTIDE-SEQUENCE OF THE FABB GENE AND IDENTIFICATION OF THE CERULENIN BINDING RESIDUE
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DOI：
10.1007/bf02983311
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发表时间：
1988-01-01
期刊：
影响因子：
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通讯作者：
VONWETTSTEINKNOWLES, P
中科院分区：
文献类型：
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作者：
KAUPPINEN, S;SIGGAARDANDERSEN, M;VONWETTSTEINKNOWLES, P
用互补的方法分离了编码β-酮酰基-ACP合成酶I的大肠杆菌FabB基因，并进行了序列测定。该酶已被纯化，并对其NH2末端残基进行了测序。活性部位的鉴定是通过~3H-蓝烯标记和该区域的无线电测序完成的。与酿酒酵母FAS2基因产物的一级结构比较，揭示了高等植物查尔酮合成酶可能的活性部位。
The fabB gene of E. coli encoding .beta.-ketoacyl-ACP synthease I has been isolated by complementation and sequenced. The enzyme has been purified and its NH2-terminal residues sequenced. Identification of the active site was accomplished by tagging with 3H-cerulenin and radio sequencing of the region. Comparison of the deduced primary structures of the fabB gene product with the FAS2 gene product of Saccharomyces cerevisiae revealed the probable active site in chalcone synthases of higher plants.