The immunofluorescent localization of regulatory and catalytic subunits of cyclic AMP-dependent protein kinase in neuronal and glial cell types of the central nervous system
The immunofluorescent localization of regulatory and catalytic subunits of cyclic AMP-dependent protein kinase in neuronal and glial cell types of the central nervous system
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中枢神经系统神经元和神经胶质细胞类型中环AMP依赖性蛋白激酶的调节和催化亚基的免疫荧光定位
DOI：
10.1016/0306-4522(81)90176-7
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发表时间：
1981
期刊：
影响因子：
3.3
通讯作者：
Alton L. Steiner
中科院分区：
文献类型：
--
作者：
Richard Cumming;Y. Koide;M. Krigman;J. Beavo;Alton L. Steiner
使用选择性和敏感的免疫荧光技术的细胞定位的调节（RI和RII）和催化成分的环腺苷酸依赖性蛋白激酶在中枢神经系统的选定区域进行了测定。神经元和神经胶质细胞的分布是相似的三个亚基，但是，每个单位表现出不同的亚细胞定位的模式。而催化单位被限制在核内位点，RI和RII被确定在细胞质和细胞核内，与RII另外显示出不同的染色核膜。RI在细胞体索马中的颗粒状分布进一步区分了这两种调节组分在该细胞区室中的细胞学定位。亚基的对比分布表明环AMP依赖性蛋白激酶的调节组分和催化组分之间可能存在功能差异，讨论了该酶在介导环AMP在中枢神经系统中的生理作用方面的作用。
The cellular localization of the regulatory (RI and RII) and catalytic components of the cyclic AMP-dependent protein kinase in selected areas of the central nervous system was determined using a selective and sensitive immunofluorescent technique. The neuronal and glial distribution was similar for the three subunits, however, each unit exhibited differences in the pattern of subcellular localization. Whereas the catalytic unit was confined to intranuclear loci, RI and RII were identified within both the cytoplasm and nucleus, with RII in addition showing distinct staining of the nuclear membrane. A granular distribution of RI in the cell soma further distinguished the cytological localization of these two regulatory components in this cellular compartment.The contrasting distribution of the subunits suggests possible functional differences between the regulatory and catalytic components of cyclic AMP-dependent protein kinase, which are discussed with regard to the role of this enzyme in mediating the physiological effects of cyclic AMP in the central nervous system.