Structure and neutrophil-activating properties of a novel inflammatory peptide (ENA-78) with homology to interleukin 8.
Structure and neutrophil-activating properties of a novel inflammatory peptide (ENA-78) with homology to interleukin 8.
复制标题
与白介素8具有同源性的新型炎症肽（ENA-78）的结构和中性粒细胞激活特性。
DOI：
10.1084/jem.174.6.1355
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发表时间：
1991-12-01
影响因子：
15.3
通讯作者：
Strieter, R M
中科院分区：
文献类型：
--
作者：
Walz, A;Burgener, R;Car, B;Baggiolini, M;Kunkel, S L;Strieter, R M
在刺激的人II型上皮细胞系A549的条件培养基中鉴定了一种新的嗜中性粒细胞活化肽，称为ENA-78。响应于用白细胞介素1 β（IL-1 β）或肿瘤坏死因子α（TNF-α）的刺激，ENA-78与IL-8、GRO α和GRO γ伴随产生和分泌。ENA-78由78个氨基酸[序列;见正文]组成，分子量为8，357。它具有与IL-8和类似物的位置相同的四个半胱氨酸，因此属于CXC肽家族。ENA-78与嗜中性粒细胞活化肽2（NAP-2）和GRO α（序列同一性分别为53%和52%）以及IL-8（22%同一性）相关。像NAP-2和GRO α一样，ENA-78刺激中性粒细胞，诱导趋化性，细胞内游离钙和胞吐作用的增加。交叉脱敏实验表明，ENA-78通过与IL-8、NAP-2和GRO α相同类型的受体起作用。
A new neutrophil-activating peptide, termed ENA-78, was identified in the conditioned media of stimulated human type II epithelial cell line A549. In response to stimulation with either interleukin 1 beta (IL-1 beta) or tumor necrosis factor alpha (TNF-alpha), ENA-78 was produced and secreted concomitantly with IL-8, GRO alpha, and GRO gamma. ENA-78 consists of 78 amino acids [sequence; see text] and has a molecular weight of 8,357. It has four cysteines positioned identically to those of IL-8 and analogues, and thus belongs to the CXC family of peptides. ENA-78 is related to neutrophil-activating peptide 2 (NAP-2) and GRO alpha (sequence identity, 53% and 52%, respectively) and IL-8 (22% identity). Like NAP-2 and GRO alpha, ENA-78 stimulates neutrophils, inducing chemotaxis, a rise in intracellular free calcium and exocytosis. Cross-desensitization experiments indicate that ENA-78 acts through the same type of receptors as IL-8, NAP-2, and GRO alpha.