Control of Spreading Depression with Electrical Fields.
Control of Spreading Depression with Electrical Fields.
复制标题
用电场控制抑郁症的传播。
DOI：
10.1038/s41598-018-26986-1
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发表时间：
2018
影响因子：
4.6
通讯作者：
Schiff,StevenJ
中科院分区：
文献类型：
--
作者：
Whalen,AndrewJ;Xiao,Ying;Kadji,Herve;Dahlem,MarkusA;Gluckman,BruceJ;Schiff,StevenJ
扩散性抑郁或去极化是一种与偏头痛、中风、出血和外伤性脑损伤相关的大规模脑病理现象。一旦启动，扩张性抑制在灰质中传播，挤压钾和其他活性分子，使细胞静息膜电化学梯度崩溃，导致spike失活和细胞肿胀，并且独立于突触传递而传播。我们利用应用于脑切片的经皮质直流电场，用本征光学成像和钾染料荧光测量，证明了扩张性抑郁的调制、抑制和预防。我们通过实验观察到一个表面正电场诱导的传播压抑传播到皮层中比未调制传播路径更深的位置，因此进一步的传播被限制和阻止，即使在电场终止后。与未调制的传播路径相比，相反的表面负电场极性会增加传播速度，并将波限制在更浅层的皮层中。这些磁场极性与阻止神经元尖峰和癫痫发作的极性相反，并且与传播抑郁症的生物物理模型一致。结果表明，电气控制和防治蔓延凹陷具有潜在的可行性。
Spreading depression or depolarization is a large-scale pathological brain phenomenon related to migraine, stroke, hemorrhage and traumatic brain injury. Once initiated, spreading depression propagates across gray matter extruding potassium and other active molecules, collapsing the resting membrane electro-chemical gradient of cells leading to spike inactivation and cellular swelling, and propagates independently of synaptic transmission. We demonstrate the modulation, suppression and prevention of spreading depression utilizing applied transcortical DC electric fields in brain slices, measured with intrinsic optical imaging and potassium dye epifluorescence. We experimentally observe a surface-positive electric field induced forcing of spreading depression propagation to locations in cortex deeper than the unmodulated propagation path, whereby further propagation is confined and arrested even after field termination. The opposite surface-negative electric field polarity produces an increase in propagation velocity and a confinement of the wave to more superficial layers of cortex than the unmodulated propagation path. These field polarities are of opposite sign to the polarity that blocks neuronal spiking and seizures, and are consistent with biophysical models of spreading depression. The results demonstrate the potential feasibility of electrical control and prevention of spreading depression.