Composition of the cell walls of several yeast species
Composition of the cell walls of several yeast species
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DOI：
10.1007/s002530051278
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发表时间：
1998-08-01
影响因子：
5
通讯作者：
Rogers, PL
中科院分区：
文献类型：
--
作者：
Nguyen, TH;Fleet, GH;Rogers, PL
细胞壁占细胞干重的26% ~ 32%，分别取自几种酵母（Kloeckera apiculata, Debaryomyces hansenii, Zygosaccharomyces bailii， Kluyveromyces marxianus和Saccharomyces cerevisiae）。用氢氧化钾在4℃下提取壁，然后用硫酸铵饱和碱溶性提取物，得到甘露蛋白、碱溶性葡聚糖和碱不溶性葡聚糖的部分。甲壳素与碱不溶性葡聚糖有关。不同菌种和菌种的菌壁内各组分所占比例不同。甘露蛋白占细胞壁的25% ~ 34%，碱不溶性葡聚糖含量在15% ~ 48%之间，碱可溶性葡聚糖含量在10% ~ 48%之间。碱溶性葡聚糖的物理外观和这些葡聚糖悬浮液的相对粘度有显著变化。这些酵母可能是具有工业和医学应用价值的多糖的新来源。
Cell walls, representing 26%-32% of the cell dry weight, were prepared from several strains of the yeasts Kloeckera apiculata, Debaryomyces hansenii, Zygosaccharomyces bailii, Kluyveromyces marxianus and Saccharomyces cerevisiae. Extraction of the walls with potassium hydroxide at 4 degrees C, followed by saturation of the alkali-soluble extract with ammonium sulphate gave fractions of mannoprotein, alkali-soluble glucan and alkali-insoluble glucan. Chitin was associated with the alkali-insoluble glucan. The proportions of the different fractions within the walls varied with the species and strain. Mannoprotein comprised between 25% and 34% of the walls, the content of alkali-insoluble glucan ranged from 15% to 48%, and the content of alkali-soluble glucan ranged from 10% to 48%. There was significant variation in the physical appearance of the alkali-soluble glucans and the relative viscosity of suspensions of these glucans. The yeasts could represent novel sources of polysaccharides with industrial and medical applications.