The Prognostic Significance of Combining High Mobility Group Box-1 and OV-6 Expression in Hepatocellular Carcinoma
The Prognostic Significance of Combining High Mobility Group Box-1 and OV-6 Expression in Hepatocellular Carcinoma
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高迁移率族Box-1和OV-6联合表达在肝细胞癌中的预后意义
DOI：
10.1007/s11427-017-9188-x
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发表时间：
2017
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炎症环境和肿瘤干细胞的存在是肝细胞癌（HCC）根治性切除术后进展和肝内复发的关键。在此，我们研究了高迁移率族蛋白1（HMGB1）表达和肝祖细胞标志物OV6在肝细胞癌中的预后意义。HMGB1和OV6的表达采用免疫组织化学分析法在包含208例HCC患者样本的组织微阵列中进行评估。HMGB1或OV6高表达的患者存在侵袭性临床或病理因素。高HMGB1水平与不良临床结局相关，并与5年复发率升高独立相关（85.5%vs.62.4%，P <0.001）。OV6阳性表达与肝癌患者的预后有关（P<0.001）。HMGB1与OV6的表达密度呈正相关（R2= 0.032，P <0.001），HMGB1的表达与HCC的预后密切相关。HMGB1的异常表达与OV6的阳性染色的组合显示比单独的单一生物标志物更差的预后性能（存活曲线下面积（AUC）=0.696）。因此，HMGB1和OV6的阳性表达可作为判断HCC预后的指标，HMGB1和OV6可能相互协同作用，预测HCC的不良预后。
The inflammatory environment and existence of cancer stem cells are critical for progression and intrahepatic recurrence of hepatocellular carcinoma (HCC) after curative resections. Here, we investigated the prognostic significance of combining high mobility group box 1 (HMGB1) expression and hepatic progenitor marker OV6 in hepatocellular carcinoma. Expression of HMGB1 and OV6 was evaluated using immunohistochemistry profiling in tissue microarrays containing samples from 208 HCC patients. Invasive clinical or pathological factors were found in patients with high expression of HMGB1 or OV6. Higher HMGB1 was associated with poorer clinical outcomes, and independently related to elevated 5-year recurrence incidence (85.5% vs. 62.4%,P<0.001). We also found that more OV6 positive staining was correlated with poor prognosis of HCC patients (P<0.001). Notably, expression of HMGB1 was positively correlated with OV6 in density (R2=0.032,P<0.001) and reversely related to HCC outcomes. Abnormal expression of HMGB1 in combination with positive staining of OV6 displayed poorer prognostic performance than single biomarker alone (area under curve (AUC) survival=0.696). Therefore, HMGB1 and OV6 positive staining are promising prognostic parameters for HCC, and we propose that HMGB1 and OV6 may cooperate with each other and predict poor prognosis of HCC.