Biodegradation of chlorinated alkanes and their commercial mixtures by Pseudomonas sp strain 273
Biodegradation of chlorinated alkanes and their commercial mixtures by Pseudomonas sp strain 273
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DOI：
10.1007/s10295-004-0186-x
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发表时间：
2006-03-01
影响因子：
3.4
通讯作者：
Bratty, MP
中科院分区：
文献类型：
--
作者：
Heath, E;Brown, WA;Bratty, MP
在好氧条件下研究了氯代烷烃的生物降解，目的是确定有利和不利的分子内氯化序列相对于所研究的酶。几个脱卤细菌菌株进行了筛选，他们的能力，降解中链多氯代烷烃以及商业混合物。在测试的生物体中，最有希望的是假单胞菌属菌株273，其具有氧解脱卤酶。碳链长度（C-6-C-16），卤素位置，和总氯含量（14- 61%w/w）的影响进行了检查，使用市售化合物和分子在我们的实验室合成。共底物，溶剂和诱导剂的影响进行了研究。纯氯代烷烃的结果表明，氯原子的相对位置强烈影响脱卤总量。最大的脱卤产率与末端氯代烷烃。α-和α，ω-氯化化合物产生了类似的结果。邻位氯化对降解的影响最为显著。当存在于分子的两端或中心时，未检测到脱卤。虽然观察到1，2-二氯癸烷的部分脱卤，但这可能是由于β-氧化和非生物机制的组合。Cereclor S52在摇瓶中仅在1，10-二氯癸烷作为共基质存在时以及通过机械乳化增加油表面积后才明显脱卤，这表明了降解商业多氯烷烃混合物的膨胀因子的重要性。
The biodegradation of chlorinated alkanes was studied under oxic conditions with the objective of identifying Favorable and unfavorable intramolecular chlorination sequences with respect to the enzymes studied. Several dehalogenating bacterial strains were screened for their ability to degrade middle-chain polychlorinated alkanes as well as a commercial mixture. Of the organisms tested, the most promising was Pseudomonas sp. strain 273, which possesses an oxygenolytic dehalogenase. The effects of carbon chain length (C-6-C-16), halogen position, and overall chlorine content (14-61% w/w) were examined using both commercially available compounds and molecules synthesized in our laboratory. The effects of co-substrates, solvents, and inducing agents were also studied. The results With pure chlorinated alkanes showed that the relative positions of the chlorine atoms strongly influenced the total amount of dehalogenation achieved. The greatest dehalogenation yields were associated with terminally chlorinated alkanes. The alpha- and alpha,omega-chlorinated compounds yielded similar results. Vicinal chlorination had the most dramatic impact on degradation. When present on both ends or at the center of the molecule, no dehalogenation was detected. Although partial dehalogenation of 1,2-dichlorodecane was observed, it was likely due to a combination of beta-oxidation and an abiotic mechanism. Cereclor S52 was appreciably dehalogenated in shake flasks only when 1, 10-dichlorodecane was present as a co-substrate and after increasing the oil surface area through mechanical emulsification, demonstrating the importance of ablotic factors in degrading commercial polychlorinated alkane mixtures.