A rapid colorimetric assay for gamma-glutamyl hydrolase (conjugase).
A rapid colorimetric assay for gamma-glutamyl hydrolase (conjugase).
复制标题
γ-谷氨酰水解酶（缀合酶）的快速比色测定。
DOI：
10.1016/0003-2697(92)90490-x
复制
发表时间：
1992
影响因子：
2.9
通讯作者：
Kalman,TI
中科院分区：
文献类型：
--
作者：
Wagh,PV;Kalman,TI
A simple procedure for the measurement of γ-glutamyl hydrolase (conjugase) activity is described. Glutamic acid released from pteroylpenta-γ-glutamate by hog kidney and chicken pancreas conjugases was quantitated using the dye 4,4′-bis(dimethylamino)benzophenone hydrazone. The procedure involves hydrolysis of the folylpoly-γ-glutamate substrate by conjugase, conversion of glutamate to α-ketoglutarate by l-glutamate dehydrogenase and colorimetric measurement of the BDBH derivative of α-ketoglutarate. The release of as little as one nmol of glutamic acid from the substrate can be measured by this procedure, which is well suited for the assay of a variety of conjugase preparations. In addition, the method should provide a general assay for the enzymatic hydrolysis of various folate and antifolate polyglutamates.
