Detection of antimicrobial peptides related to piscidin 4 in important aquacultured fish
Detection of antimicrobial peptides related to piscidin 4 in important aquacultured fish
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DOI：
10.1016/j.dci.2009.11.004
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发表时间：
2010-03-01
影响因子：
2.9
通讯作者：
Noga, Edward J.
中科院分区：
文献类型：
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作者：
Corrales, Jone;Mulero, Ivan;Noga, Edward J.
鱼的上皮表面，如肠道、皮肤和鳃，包括可能的病原体入侵的大表面积。抗菌肽（Antimicrobial Peptides，AMPS）是鱼类天然免疫的重要组成部分，对鱼类具有重要的保护作用. Piscidin是AMP家族。本研究通过昆虫印迹、Western印迹、ELISA和免疫组织化学等方法，对条纹鲈鱼（Morone saxatilis）、白色鲈鱼（M. chrysops）、欧洲鲈鱼（Dicentrarchus labrax）、金头鲷（Sparus aurata）、红鼓鱼（Sciaenops ocellatus）和肺鱼（Lates calcarifer）。通过细菌印迹，所有物种的鳃提取物具有抗菌活性，对应于鱼杀菌素4的迁移率。Western blotting结果表明，鱼杀菌素4在鲈鱼鳃组织中的免疫反应性最强。通过ELISA在条纹鲈鱼鳃中检测到的鱼杀菌素4的浓度（类似于20 μ g/ml）完全在抑制重要的鱼类细菌病原体的水平内。除肺鱼外，所有鱼类均通过免疫组化检测到Piscidin 4。Piscidin 4阳性细胞被鉴定为肥大细胞（MC），但并非所有MC都是Piscidin 4阳性的。物种、年龄、大小和取样时的生理条件是影响不同物种piscidin表达的因素。我们的数据提供了强有力的证据表明，鱼杀菌素4亚型存在于所有这些商业上重要的物种。(C)2009爱思唯尔有限公司保留所有权利。
Epithelial surfaces of fish, such as the gut, skin and gills, comprise a large surface area for possible pathogen invasion. Antimicrobial peptides (AMPS), innate immunity components, play a significant role in protecting fish. Piscidins are a family of AMPs. In this study, we detected the presence of the recently discovered piscidin 4 via bug blot, Western blot, ELISA and/or immunohistochemistry in striped bass (Morone saxatilis), white bass (M. chrysops), European seabass (Dicentrarchus labrax), gilthead seabream (Sparus aurata), red drum (Sciaenops ocellatus), and barramundi (Lates calcarifer). Via bug blot, gill extracts from all species had antibacterial activity corresponding to the migration rate of piscidin 4. Western blotting showed that piscidin 4 immunoreactivity was greatest in striped bass gill extract. The concentrations of piscidin 4 detected by the ELISA in striped bass gill (similar to 20 mu g/ml) were well within the levels that are inhibitory to important fish bacterial pathogens. Piscidin 4 was also detected via immunohistochemistry in all fish except barramundi. Piscidin 4-positive cells were identified as mast cells (MC), but not all MC were piscidin 4-positive. Species, age, size and physiological condition at sampling were some factors that might affect piscidin expression in different species. Our data provide strong evidence that piscidin 4 isoforms are present in all these commercially important species. (C) 2009 Elsevier Ltd. All rights reserved.