Xylanase production by Trichoderma reesei Rut C-30 grown on L-arabinose-rich plant hydrolysates.
Xylanase production by Trichoderma reesei Rut C-30 grown on L-arabinose-rich plant hydrolysates.
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DOI：
10.1016/j.biortech.2004.08.007
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发表时间：
2005-05
影响因子：
11.4
通讯作者：
H. Xiong;N. von Weymarn;O. Turunen;M. Leisola;O. Pastinen
中科院分区：
文献类型：
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作者：
H. Xiong;N. von Weymarn;O. Turunen;M. Leisola;O. Pastinen
研究了富含L-阿拉伯糖的植物水解物作为碳源和木聚糖酶诱导剂对里氏木霉Rut C-30产木聚糖酶的影响。在燕麦壳和甜菜浆水解物上培养的木聚糖酶活性显著高于在乳糖上培养的。在分批培养中，以燕麦壳水解液和乳糖为底物，木聚糖酶活力（约510 IU/ml）比以乳糖为底物（约60 IU/ml）高9倍。以甜菜浆水解液和乳糖为底物进行分批培养，木聚糖酶活力可达630 IU/ml。以燕麦壳水解液-乳糖为底物进行流加培养，培养4天后木聚糖酶活力可达1350 IU/ml。T.与在乳糖上培养相比，在两种水解产物上培养里氏杆菌。此外，木聚糖酶I-III的相对量是相似的，与所使用的碳源无关。
The suitability of L-arabinose-rich plant hydrolysates as carbon sources and inducers of xylanase production in Trichoderma reesei Rut C-30 was tested. Significantly higher xylanase activities were obtained in cultures on oat husk and sugar beet pulp hydrolysates than on lactose. In batch culture with oat husk hydrolysate and lactose, the xylanase activity was about 9 times higher (∼510IU/ml) than in lactose (∼60 IU/ml). Even higher xylanase activity (∼630IU/ml) was obtained when the batch cultivations were done on sugar beet pulp hydrolysate and lactose. In a fed-batch culture using oat husk hydrolysate–lactose the xylanase activity was as high as 1350IU/ml in 4 days. The cellulase production clearly decreased when T. reesei was cultured on both hydrolysates compared to the cultivation on lactose. Moreover, the relative amounts of the xylanases I–III were similar regardless the used carbon source.