Developing enhanced magnetoimmunosensors based on low-cost screen-printed electrode devices
Developing enhanced magnetoimmunosensors based on low-cost screen-printed electrode devices
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DOI：
10.1515/revac-2016-0004
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发表时间：
2016-07-01
影响因子：
4.3
通讯作者：
Baldrich, Eva
中科院分区：
文献类型：
--
作者：
Herrasti, Zorione;de la Serna, Erica;Baldrich, Eva
电化学磁免疫传感器结合了许多问题，保证了极高的性能，并与复杂样品矩阵的研究兼容。首先，分析物免疫捕获利用了抗体的高亲和力和特异性。其次，磁性颗粒(MP)提供了比结合在二维结构上、从非目标样品组分分离和目标分析物浓缩更快和更有效的免疫捕获。最后，电化学检测使用坚固且可能小型化的测量设备和传感器提供灵敏度和快速信号生成。相反，与传统生物传感器的操作相比，MP处理对最终用户来说稍微复杂一些，并且更难集成到护理设备中。已经尝试使免疫磁结合自动化，第一批用于磁免疫分析的荧光和分光光度检测的自动化系统和平台已经进入市场。在现有的不同类型的电极中，丝网印刷电极(SPE)因其生产成本低、可接受的性能和设备间的重复性而脱颖而出，这使它们成为分析应用的极佳选择。此外，每个固相萃取需要一个压印在平面物理衬底上的整个电化学池，这使得它可以在小体积内进行检测，特别有利于MP的磁约束和微流控结构的集成。在本文中，我们讨论了用固相萃取和MP生产电化学磁免疫传感器的优点和成功开发这种器件的线索。然后我们修改一些在文学作品中发表的最优秀的作品。
Electrochemical magnetoimmunosensors combine a number of issues that guarantee extremely high performance and also compatibility with the study of complex sample matrices. First, analyte immunocapture exploits the high affinity and specificity of antibodies. Second, magnetic particles (MP) provide faster and more efficient immunocapture than binding on two-dimensional structures, separation from nontarget sample components, and concentration of the target analyte. Finally, electrochemical detection supplies sensitivity and fast signal generation using robust and potentially miniaturized measurement equipment and transducers. On the contrary, MP handling is slightly more complex for end-users and more difficult to integrate in point-of-care devices than the manipulation of a classical biosensor. Attempts have been made to automate immunomagnetic binding, and the first robotized systems and platforms for the fluorescent and spectrophotometric detection of magnetoimmunoassays have already reached the market. Among the different types of electrodes available, screen-printed electrodes (SPE) stand out because of their low production cost and yet acceptable performance and interdevice reproducibility, which make them an excellent choice for analytical applications. In addition, each SPE entails a whole electrochemical cell stamped on a planar physical substrate, which makes it possible detection in small volumes and is especially favorable for the magnetic confinement of MP and the integration of microfluidic structures. In this article, we discuss the advantages obtained by using SPE and MP for the production of electrochemical magnetoimmunosensors and the clues for the successful development of such devices. We then revise some of the most outstanding works published in the literature.