The wheat HMW-glutenin 1Dy10 gene promoter controls endosperm expression in Brachypodium distachyon.
The wheat HMW-glutenin 1Dy10 gene promoter controls endosperm expression in Brachypodium distachyon.
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DOI：
10.4161/gmcr.27371
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发表时间：
2014-01
期刊：
影响因子：
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通讯作者：
Blechl AE
中科院分区：
文献类型：
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作者：
Thilmony R;Guttman ME;Lin JW;Blechl AE
禾本科植物二穗短柄草（Brachypodium distachyon）已成为温带禾本科植物基因结构和功能研究的模式系统。作为第一个演示的效用短柄草研究小麦基因启动子功能，我们转化了T-DNA，其中包括uidA报告基因控制下的小麦高分子量谷蛋白亚基（HMW-GS）基因启动子和转录终止子。为了比较，通过基因枪将相同的表达盒导入小麦中。GUS组织化学染色结果表明，小麦启动子在所有短柄草和小麦纯合植株的胚乳中都得到了高效表达。它在转基因小麦的任何其他组织中没有活性，但在四个纯合转基因短柄草系的雌蕊花柱中表现出可变的和零星的活性。获得转基因短柄草属植物的容易性和小麦HMW-GS启动子在这些植物中表达的整体忠实性使得该模型系统可能可用于来自难以转化的谷类作物物种的其他启动子的研究。
The grass species Brachypodium distachyon has emerged as a model system for the study of gene structure and function in temperate cereals. As a first demonstration of the utility of Brachypodium to study wheat gene promoter function, we transformed it with a T-DNA that included the uidA reporter gene under control of a wheat High-Molecular-Weight Glutenin Subunit (HMW-GS) gene promoter and transcription terminator. For comparison, the same expression cassette was introduced into wheat by biolistics. Histochemical staining for β-glucuronidase (GUS) activity showed that the wheat promoter was highly expressed in the endosperms of all the seeds of Brachypodium and wheat homozygous plants. It was not active in any other tissue of transgenic wheat, but showed variable and sporadic activity in a minority of styles of the pistils of four homozygous transgenic Brachypodium lines. The ease of obtaining transgenic Brachypodium plants and the overall faithfulness of expression of the wheat HMW-GS promoter in those plants make it likely that this model system can be used for studies of other promoters from cereal crop species that are difficult to transform.