Bacterial Enzyme Responsive Polymersomes: A Closer Look at the Degradation Mechanism of PEG-block-PLA Vesicles
Bacterial Enzyme Responsive Polymersomes: A Closer Look at the Degradation Mechanism of PEG-block-PLA Vesicles
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DOI：
10.1071/ch13527
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发表时间：
2014-04
影响因子：
1.1
通讯作者：
Katrin‐Stephanie Tücking;Stephan Handschuh‐Wang;H. Schönherr
中科院分区：
文献类型：
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作者：
Katrin‐Stephanie Tücking;Stephan Handschuh‐Wang;H. Schönherr
本文报道了被病原菌的酶选择性切割的刺激响应性聚合物囊泡。通过荧光寿命成像显微镜监测了填充有报告染料的聚乙二醇-嵌段聚乳酸（PEG-b-PLA）聚合物囊泡中聚酯壁因蛋白酶K的酶促降解而发生的局部破坏。胶囊打开发生在数分钟至数小时的时间尺度上，导致染料释放，随后是缓慢的胶囊崩解，最后在数周内裂解成单体乳酸。这些纳米容器代表了新型治疗诊断系统中的一个有前途的元素，可用于先进的伤口敷料。
Stimuli-responsive polymersomes that are selectively cleaved by enzymes of pathogenic bacteria are herein reported. The local disruption of the polyester wall in poly(ethylene glycol)-block-poly(lactic acid) (PEG-b-PLA) polymersomes filled with reporter dyes owing to enzymatic degradation by proteinase K was monitored by fluorescence lifetime imaging microscopy. Capsule opening occurred on timescales of minutes to hours, leading to the release of the dye, followed by a slow capsule disintegration, and finally cleavage into monomeric lactic acid over several weeks. These nanocontainers represent a promising element in novel theranostic systems for potential application in advanced wound dressings.