Effects of water deprivation and rehydration on c-Fos and FosB staining in the rat supraoptic nucleus and lamina terminalis region
Effects of water deprivation and rehydration on c-Fos and FosB staining in the rat supraoptic nucleus and lamina terminalis region
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DOI：
10.1152/ajpregu.00399.2004
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发表时间：
2005-01-01
影响因子：
2.8
通讯作者：
Cunningham, JT
中科院分区：
文献类型：
--
作者：
Ji, LL;Fleming, T;Cunningham, JT
本实验研究了缺水(24 h，n=11；48 h，n=12)和缺水加复水(22 h+水，n=11；46 h+水，n=10)后，成年雄性大鼠视上核(SON)、终板血管器(OVLT)和视前正中核(MnPO)内CFos和FosB的表达。对照组大鼠(n=15)自由饮水。分别取自每个大脑的连续切片进行免疫细胞化学处理，使用针对c-Fos或FosB蛋白的初级抗体。测定不同组别(n=6-7)的血浆渗透压、加压素、红细胞压积和血浆蛋白。缺水24、48h后SON内c-Fos阳性细胞数显著增加。相比之下，补液组与对照组没有区别。缺水显著增加OVLT和MnPO的c-Fos染色，但复水不改变c-Fos染色。仅在缺水48h时SON中FosB的染色显著增加，而复水可显著降低这种影响。在MnPO和OVLT中，FosB染色在缺水后显著增加，与c-Fos染色一样，这些增加不受复水的影响。缺水显著增加渗透压和红细胞压积，以及血浆蛋白和加压素浓度。复水大鼠的血浆测量结果与对照组没有差别。我们得出结论，缺水和复水对c-Fos和FosB染色的影响具有区域依赖性。
We studied cFos and FosB staining in the supraoptic nucleus (SON) the organum vasculosum of the lamina terminalis (OVLT) and the median preoptic nucleus (MnPO) in adult male rats after water deprivation (24 h, n = 11; 48 h, n = 12) and water deprivation with rehydration (22 h + water, n = 11; 46 h + water, n = 10). Control rats (n = 15) had water available ad libitum. Separate sets of serial sections from each brain were processed for immunocytochemistry using primary antibodies against either c-Fos or FosB protein. Plasma osmolality, vasopressin, hematocrit, and plasma proteins were measured in separate groups (n = 6-7). The number of c-Fos-positive cells in the SON was significantly increased after 24 and 48 h of water deprivation. In contrast, rehydrated groups were not different from control. Water deprivation significantly increased c-Fos staining in both the OVLT and the MnPO, but c-Fos staining was not altered by rehydration. FosB staining in the SON was significantly increased only by 48-h water deprivation, and this effect was significantly decreased by rehydration. In the MnPO and OVLT, FosB staining was significantly increased by water deprivation, and, like c-Fos staining, these increases were not affected by rehydration. Water deprivation significantly increased osmolality and hematocrit, as well as plasma protein and vasopressin concentrations. Plasma measurements from rehydrated rats were not different from control. We conclude that water deprivation and rehydration differentially affect c-Fos and FosB staining in a region-dependent manner.