APHID STYLET ACTIVITIES DURING POTYVIRUS ACQUISITION FROM PLANTS AND AN IN-VITRO SYSTEM THAT CORRELATE WITH SUBSEQUENT TRANSMISSION
APHID STYLET ACTIVITIES DURING POTYVIRUS ACQUISITION FROM PLANTS AND AN IN-VITRO SYSTEM THAT CORRELATE WITH SUBSEQUENT TRANSMISSION
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DOI：
10.1007/bf01874855
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发表时间：
1995-07-01
影响因子：
1.8
通讯作者：
HARDIE, J
中科院分区：
文献类型：
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作者：
POWELL, G;PIRONE, T;HARDIE, J
与收购烟草蚀纹马铃薯Y病毒饥饿桃蚜在单一的，电子记录的植物或Parafilm膜渗透的行为事件进行了比较。29%的蚜虫从植物上获得病毒，然后传播到试验植物。探针穿刺质膜，由潜在的下降（PD）的细胞内信号电压水平，发生在84%的渗透，和病毒传播总是与此行为事件在收购。细胞内探针尖端定位的周期，称为PD阶段II，范围从3.6- 12.2秒，并且总是包括三个连续的子阶段，指定为II 1，II 2和II 3。百分之九十六的pds包括不同的脉冲在阶段II 3。一个波形，非常类似于这些脉冲产生的59%的蚜虫，探测病毒悬浮液通过Parafilm膜; 19%的蚜虫随后传输膜获得性病毒和传输显着相关的发生时相II 3样脉冲在收购。记录阶段II 3脉冲发生的持续时间，无论是在植物上或在体外系统中，没有影响蚜虫的病毒传播效率。从一个特定的行为活动的水悬浮液中的病毒摄取的关联进行了讨论，作为证据的“摄入-排泄”假说的非持久性传播。饥饿的蚜虫从感染的叶片组织或体外系统中获得病毒的传播效率显着高于非饥饿的蚜虫。饥饿和非饥饿的昆虫被电记录穿透人工膜，并且在传输效率上再次存在明显差异（饥饿的蚜虫，26%;非饥饿的蚜虫，2%）。饥饿昆虫的传播效率较高，不能解释的行为差异，结果支持的假设，即非行为因素决定增强马铃薯Y病毒传播收购前饥饿。
The behavioural events associated with acquisition of tobacco etch potyvirus by starved Myzus persicae during single, electrically-recorded penetrations of plants or a Parafilm membrane were compared. Twenty nine percent of aphids acquired virus from plants and subsequently transmitted to test plants. Stylet puncture of the plasmalemma, indicated by a potential drop (pd) to the intracellular signal voltage level, occurred during 84% of penetrations, and virus transmission was always associated with this behavioural event during acquisition. Periods of intracellular stylet tip location, known as pd phase II, ranged from 3.6-12.2s, and always comprised three consecutive sub-phases, designated II1, II2 and II3. Ninety six percent of pds included distinct pulses during phase II3. A waveform which closely resembled these pulses was produced by 59% of aphids that probed a virus suspension through a Parafilm membrane; nineteen percent of the aphids subsequently transmitted membrane-acquired virus and transmission was significantly associated with the occurrence of the phase II3-like pulses during acquisition. The duration of occurrence of recorded phase II3 pulses, either on plants or the in vitro system, did not influence the virus transmission efficiency of aphids. The association of virus uptake from aqueous suspension with a particular behavioural activity is discussed as evidence for the 'ingestion-egestion' hypothesis for non-persistent transmission. Starved aphids acquiring virus from infected leaf tissue or the in vitro system had significantly higher transmission efficiencies than non-starved aphids. Starved and non-starved insects were electrically-recorded penetrating the artificial membrane, and again there was a clear difference in transmission efficiency (starved aphids, 26%; non-starved aphids, 2%). The higher transmission efficiency of starved insects could not be explained by behavioural differences, and the results lend support to the hypothesis that non-behavioural factors determine the enhancement of potyvirus transmission by pre-acquisition starvation.