Reduced sulfur compound oxidation by Thiobacillus caldus
Reduced sulfur compound oxidation by Thiobacillus caldus
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DOI：
10.1128/jb.178.1.6-11.1996
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发表时间：
1996-01-01
影响因子：
3.2
通讯作者：
Lindstrom, EB
中科院分区：
文献类型：
--
作者：
Hallberg, KB;Dopson, M;Lindstrom, EB
利用中等嗜热性喜温硫杆菌KU菌株的静息细胞研究了还原型无机硫化合物的氧化。氧消耗率和总氧消耗量测定的还原硫化合物硫代硫酸盐，连四硫酸盐，硫，硫化物，和亚硫酸盐在不存在和存在下的抑制剂和解偶联剂。解偶联剂2，4-二硝基苯酚和羰基氰间氯苯腙对硫代硫酸盐的氧化没有影响，表明硫代硫酸盐是在细胞质周代谢的。相反，解偶联剂完全抑制连四硫酸盐、硫化物、硫和亚硫酸盐的氧化，表明这些化合物在T.卡尔杜斯大学N-乙基马来酰亚胺在元素硫阶段抑制连四硫酸盐和硫代硫酸盐的氧化，而2-庚基-4-羟基喹啉-N-氧化物在亚硫酸盐阶段抑制硫代硫酸盐、连四硫酸盐和元素硫的氧化。通过使用解偶联剂和抑制剂，发现硫代硫酸盐被氧化成连四硫酸盐，在连四硫酸盐和硫化物的氧化过程中形成元素硫，亚硫酸盐是连四硫酸盐和硫代谢的中间体。根据这些数据，我们提出了一个模型的代谢还原无机硫化合物的T。卡尔杜斯大学
The oxidation of reduced inorganic sulfur compounds was studied by using resting cells of the moderate thermophile Thiobacillus caldus strain KU. The oxygen consumption rate and total oxygen consumed were determined for the reduced sulfur compounds thiosulfate, tetrathionate, sulfur, sulfide, and sulfite in the absence and in the presence of inhibitors and uncouplers. The uncouplers 2,4-dinitrophenol and carbonyl cyanide m-chlorophenyl-hydrazone had no affect on the oxidation of thiosulfate, suggesting that thiosulfate is metabolized periplasmically. In contrast, the uncouplers completely inhibited the oxidation of tetrathionate, sulfide, sulfur, and sulfite, indicating that these compounds are metabolized in the cytoplasm of T. caldus KU. N-Ethylmaleimide inhibited the oxidation of tetrathionate and thiosulfate at the stage of elemental sulfur, while 2-heptyl-4-hydroxyquinoline-N-oxide stopped the oxidation of thiosulfate, tetrathionate, and elemental sulfur at the stage of sulfite. The following intermediates in the oxidation of the sulfur compounds were found by using uncouplers and inhibitors: thiosulfate was oxidized to tetrathionate, elemental sulfur was formed during the oxidation of tetrathionate and sulfide, and sulfite was found as an intermediate of tetrathionate and sulfur metabolism. On the basis of these data we propose a model for the metabolism of the reduced inorganic sulfur compounds by T. caldus KU.