Relationship of the cAMP-dependent protein kinase pathway to the SNF1 protein kinase and invertase expression in Saccharomyces cerevisiae.
Relationship of the cAMP-dependent protein kinase pathway to the SNF1 protein kinase and invertase expression in Saccharomyces cerevisiae.
复制标题
酿酒酵母中 cAMP 依赖性蛋白激酶途径与 SNF1 蛋白激酶和转化酶表达的关系。
DOI：
10.1093/genetics/130.1.71
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发表时间：
1992
期刊：
影响因子：
3.3
通讯作者：
Carlson,M
中科院分区：
文献类型：
--
作者：
Hubbard,EJ;Yang,XL;Carlson,M
SNF1 蛋白激酶和相关的 SNF4 蛋白是酿酒酵母中葡萄糖抑制释放所必需的。为了鉴定功能相关的蛋白质，我们选择了多拷贝抑制 snf4 突变体棉子糖生长缺陷的基因。在回收的 9 个基因中，有两个来自 cAMP 依赖性蛋白激酶 (cAPK) 途径，MSI1 和 PDE2。这些基因剂量的增加部分补偿了 snf1 和 snf4 无效突变体中养分利用和孢子形成的缺陷，但不能恢复转化酶表达。这些结果表明 SNF1 和 cAPK 影响一些相同的细胞对营养物质的反应。为了检查 cAPK 通路在转化酶调节中的作用，我们分析了 cAPK 不受 cAMP 调节的突变体。转化酶的表达受到葡萄糖反应的调节，并且依赖于 SNF1 功能。因此，cAMP 响应性 cAPK 对于转化酶的调节是可有可无的。
The SNF1 protein kinase and the associated SNF4 protein are required for release of glucose repression in Saccharomyces cerevisiae. To identify functionally related proteins, we selected genes that in multicopy suppress the raffinose growth defect of snf4 mutants. Among the nine genes recovered were two genes from the cAMP-dependent protein kinase (cAPK) pathway, MSI1 and PDE2. Increased dosage of these genes partially compensates for defects in nutrient utilization and sporulation in snf1 and snf4 null mutants, but does not restore invertase expression. These results suggest that SNF1 and cAPK affect some of the same cellular responses to nutrients. To examine the role of the cAPK pathway in regulation of invertase, we assayed mutants in which the cAPK is not modulated by cAMP. Expression of invertase was regulated in response to glucose and was dependent on SNF1 function. Thus, a cAMP-responsive cAPK is dispensable for regulation of invertase.