Raman spectroscopy studies for diagnosis of cancers in human uterine cervix
Raman spectroscopy studies for diagnosis of cancers in human uterine cervix
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DOI：
10.1016/j.vibspec.2006.01.011
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发表时间：
2006-05-30
影响因子：
2.5
通讯作者：
Kartha, VB
中科院分区：
文献类型：
--
作者：
Krishna, CM;Prathima, NB;Kartha, VB
宫颈癌是发达国家和发展中国家妇女的主要癌症之一。光谱学方法主要是傅里叶变换红外（FTIR）和荧光技术，已广泛应用于宫颈癌的细胞和组织诊断。在指纹区记录正常组织和恶性组织的拉曼光谱。正常宫颈组织的特征是强而宽的酰胺1，宽的酰胺III和853和938 cm（-1）处的强峰，这可归因于结构蛋白，如胶原蛋白。与正常组织相比，恶性组织光谱的突出特征是酰胺1相对较弱和更清晰，δ CH2的红移较小，酰胺III更清晰，这表明存在脱氧核糖核酸（DNA）、脂质和非胶原蛋白。为了开发高度客观的判别方法，使用主成分分析（PCA）进行了非常详细的数据分析。制备正常和恶性标准组并进行回顾性和前瞻性检测。利用因子分数、马氏距离和光谱残差等参数进行判别，实现了正常光谱和恶性光谱的清晰聚类。为了得到明确的判别，还考虑了结合上述所有判别参数的多参数方法（极限检验）。该分析产生了非常高的灵敏度和特异性，达到99.5%。本研究结果验证了拉曼光谱法在宫颈癌正常组织和恶性组织鉴别中的应用。(C) 2006 Elsevier B.V.版权所有
Cancer of uterine cervix is one of the leading cancers among women in both developed and developing countries. Optical spectroscopy methods mostly Fourier transform infrared (FTIR) and fluorescence have widely been used to diagnose cervix cancer using cells as well as tissues. Raman spectra of normal and malignant tissues were recorded in fingerprint region. Normal cervix tissues are characterized by strong, broad amide 1, broader amide III and strong peaks at 853 and 938 cm(-1) which can be attributed to structural proteins such as collagen. Prominent features of malignant tissue spectra with respect to normal tissue are-relatively weaker and sharper amide 1, minor red shift in Delta CH2 and sharper amide III which indicate the presence of Deoxyribonucleic acid (DNA), lipids and non-collagenous proteins. In order to develop highly objective discrimination methods, very elaborate data analysis was carried out using Principal Components Analysis (PCA). Standard sets for normal and malignant were prepared and tested retrospectively and prospectively. Several parameters such as (scores of factor, Mahalanobis distance and spectral residuals) were explored for discrimination and very clean clustering of normal and malignant spectra was achieved. A multiparametric approach (limit test) combining all the above discrimination parameters was also considered, in order to develop unambiguous discrimination. This analysis has produced very high, 99.5%, sensitivity and specificity. Results obtained in this study thus validate Raman spectroscopy methods for discrimination of normal and malignant tissues in cervical cancers. (C) 2006 Elsevier B.V. All rights reserved.