Synthesis of and triplex formation in oligonucleotides containing 2’-deoxy-6-thioxanthosine
Synthesis of and triplex formation in oligonucleotides containing 2’-deoxy-6-thioxanthosine
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含有 2-脱氧-6-硫黄苷的寡核苷酸的合成和三链体形成
DOI：
10.1016/j.bmc.2018.06.004
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Akihiro Ohkubo
中科院分区：
文献类型：
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作者：
akeshi Inde;Shuhei Nishizawa;Yuusaku Hattori;Takashi Kanamori;Hideya Yuasa;Kohji Seio;Mitsuo Sekine;Akihiro Ohkubo
本研究旨在合成含有2‘-脱氧-6-硫代黄嘌呤(S6X)和2’-脱氧-6-硫代鸟苷(S6Gs)残基的三链形成寡核苷酸(TFOS)，并检测其形成三链的能力。与未修饰的TFOS相比，S6X和S6GS残基的连续排列使寡核苷酸的三链形成能力提高了50倍以上。此外，尽管s6X可以通过s6X的互变异构化形成s6X-GC错配对，但含有错配对的三链的稳定性远低于完全匹配的三链。本研究结果表明，含有s6Xs和s6Gs残基的修饰TFOS具有优良的性质，有望用于基因治疗和DNA纳米技术。
This study aimed to synthesize triplex-forming oligonucleotides (TFOs) containing 2′-deoxy-6-thioxanthosine (s6X) and 2′-deoxy-6-thioguanosine (s6Gs) residues and examined their triplex-forming ability. Consecutive arrangement of s6X and s6Gs residues increased the triplex-forming ability of the oligonucleotides more than 50 times, compared with the unmodified TFOs. Moreover, the stability of triplex containing a mismatched pair was much lower than that of the full-matched triplex, though s6X could form a s6X-GC mismatched pair via tautomerization of s6X. The present results reveal excellent properties of modified TFOs containing s6Xs and s6Gs residues, which may be harnessed in gene therapy and DNA nanotechnology.