Transformation in vitro of [4-14-C]-dehydroepiandrosterone into 7-oxygenated derivatives by normal human male and female skin tissue.
Transformation in vitro of [4-14-C]-dehydroepiandrosterone into 7-oxygenated derivatives by normal human male and female skin tissue.
复制标题
正常人类男性和女性皮肤组织将[4-14-C]-脱氢表雄酮体外转化为7-氧化衍生物。
DOI：
10.1038/jid.1969.61
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发表时间：
1969
期刊：
影响因子：
--
通讯作者：
M. Julesz
中科院分区：
文献类型：
--
作者：
I. Faredin;A. Fazekas;I. Toth;K. Kókai;M. Julesz
先前显示，人皮肤切片在体外强烈代谢[4- 14 C]-脱氢表雄酮（[4- 14 C]-DHA）（1-3）。从众多的代谢产物雄甾-4-烯-3，17-二酮，雄甾酮和睾酮的分离和鉴定。研究表明，DHA代谢的主要途径之一是通过雄甾-4-伊利-3，17-二酮向雄甾酮和睾酮转化（3，4）。该代谢途径通过与[4 °C]-雄甾-4-烯-3，17-二酮（5）孵育得到证实。通过[4-°C]-DHA与皮肤切片的反复交换，也以相当大的产率形成强极性代谢物，即7-酮-脱氢表雄酮（7-酮-DHA）和7 α-羟基-脱氢表雄酮（7 α-OH-DHA）（2，3）。由于7-氧合衍生物的形成似乎是DHA在人体皮肤中的另一种代谢途径，我们通过孵育[4.. 14 C]-DHA与取自正常男性和女性患者的腹部皮肤样品进行比较，以获得关于该第二代谢产物的显著性的仅仅定性的数据。
It was previously shown that human skin slices intensively metabolized [4-14C]-dehydroepiandrosterone ([4-'°C]-DHA) in vitro (1—3). From the numerous metabolites androst-4-ene3, 17-dione, androsterone and testosterone were isolated and identified. It was suggested that one of the main pathways of DHA metabolism proceeded towards androsterone and testosterone via androst-4-erie-3,17-dione (3, 4). This metabolic pathway was confirmed by incubations with [4-°°C]-androst-4-ene-3, 17-dione (5). By iricubations of [4-°C]-DHA with skin slices strongly polar metabolites i.e. 7-keto-dehydroepiandrosterone (7-keto-DHA) and 7ahydroxy-dehydroepiandrosterone (7a-OH-DHA) were also formed in considerable yields (2, 3). Since the formation of 7-oxygenated derivatives appears to be another metabolic pathway of DHA in the human skin we studied the synthesis of these steroids by incubating [4..'4C]-DHA with abdominal skin samples taken from normal male and female patients in order to obtain merely qualitative data concerning the significance of this second metabolic