Insecticide resistance status of Myzus persicae (Hemiptera: Aphididae) populations from peach and tobacco in mainland Greece
Insecticide resistance status of Myzus persicae (Hemiptera: Aphididae) populations from peach and tobacco in mainland Greece
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DOI：
10.1002/ps.1409
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发表时间：
2007-08-01
影响因子：
4.1
通讯作者：
Tsitsipis, John A.
中科院分区：
文献类型：
--
作者：
Margaritopoulos, John T.;Skouras, Panagiotis J.;Tsitsipis, John A.
采用FAO浸渍法测定了88份和38份田间桃蚜样品对吡虫啉和溴氰菊酯的敏感性。从2004年至2006年期间，从希腊各地区的烟草和桃子中收集了田间样品。此外，497，349和370来自桃和烟草的克隆筛选三个已知的抗性机制，升高的酯酶，修改乙酰胆碱酯酶（MACE）和敲低抗性（kdr），分别使用生化和DNA诊断。大多数用吡虫啉检测的样本均显示出低抗性因子（P. F）-39%低于5，21%在5至10之间。然而，9%的样品（均来自烟草）显示出相对较高的RF值（24-73）。作物之间的差异，较高的RF值记录在烟草样品比桃。溴氰菊酯的生物测定显示，在检查的人口的发展强烈的阻力。RF大多数高于23，在29%的样本中，RF极高（152-436）。最后，三种已知的抗性机制被发现在高频率的人口调查，虽然作物和年份之间的一些差异进行了检测。本研究对M. persicae讨论。(c)2007化学工业协会。
The susceptibility of 88 and 38 field samples of Myzus persicae (Sulzer) to imidacloprid and deltamethrin respectively was examined using the FAO dip test bioassay. The field samples were collected from tobacco and peach from various regions of Greece in the period from 2004 to 2006. In addition, 497, 349 and 370 clones originating from peach and tobacco were screened for the three known resistance mechanisms, elevated esterases, modified acetylcholinesterase (MACE) and knockdown resistance (kdr) respectively, using biochemical and DNA diagnostics. Most of the samples assayed with imidacloprid showed low resistance factors (P.Fs)-39% below 5 and 21% between 5 and 10. However, 9% of the samples (all from tobacco) showed relatively high RF values (24-73). Differences were found between crops, with higher RF values recorded in samples from tobacco than in those from peach. Bioassays with deltamethrin revealed the development of strong resistance in the populations examined. The RFs were mostly higher than 23, and in 29% of the samples they were extremely high (152-436). Finally, the three known resistance mechanisms were found in high frequencies in the populations examined, although some differences between crops and years were detected. The implications of the study for management schemes against M. persicae are discussed. (c) 2007 Society of Chemical Industry.