Analyses of WOX4 transgenics provide further evidence for the evolution of the WOX gene family during the regulation of diverse stem cell functions
Analyses of WOX4 transgenics provide further evidence for the evolution of the WOX gene family during the regulation of diverse stem cell functions
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DOI：
10.4161/psb.5.7.12104
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发表时间：
2010-01-01
影响因子：
2.9
通讯作者：
Scanlon, Michael J.
中科院分区：
文献类型：
--
作者：
Ji, Jiabing;Shimizu, Rena;Scanlon, Michael J.
WOX（WUSCHEL-RELATED HOMEOBOX）基因家族由15个植物特异性转录因子组成，在植物发育中发挥重要作用。遗传、系统发育和基因组学分析表明，WOX基因通常非自主地在植物分生组织和器官原基中组织干细胞和初始细胞群体。以前的交叉互补分析表明，不同的WOX旁系同源物的功能多样性可以解释很大程度上由启动子进化，虽然旁系同源物特异性蛋白质：：蛋白质相互作用也牵连。最近的报道描述了WOX 4在原形成层发育过程中的功能，原形成层包括植物脉管系统的分生组织。在这里，我们表明，WOX 4未能补充PRS 1/WOX 3的功能时，从PRS 1/WOX 3天然启动子驱动。这些数据表明，WOX 4确定不同的DNA目标和/或相互作用的蛋白质在发展过程中的脉管系统原形成层比PRS 1/WOX 3在指定的侧器官初始细胞。在番茄中由SlWOX 4直系同源物的过表达诱导的超复合叶表型的鉴定表明了维管图案和叶复杂性之间的功能联系。
The WOX (WUSCHEL-RELATED HOMEOBOX) gene family of Arabidopsis comprises fifteen plantspecific transcriptional factors that play important development roles. Genetic, phylogenetic and genomic analyses suggest that WOX genes generally act nonautonomously to organize stem-cell and initial-cell populations within plant meristems and organ anlagen. Previous cross-complementation analyses indicate that the functional diversification of distinct WOX paralogs may be explained largely by promoter evolution, although paralog-specific protein:: protein interactions are also implicated. A recent report described WOX4 function during development of the procambium, which comprises the meristematic tissues of the plant vasculature. Here we show that WOX4 fails to complement PRS1/WOX3 function, when driven from the PRS1/WOX3 native promoter. These data suggest that WOX4 identifies different DNA targets and/or interacting proteins during development of the vasculature procambium than does PRS1/WOX3 during the specification of lateral organ initial cells. The identification of super-compound leaf phenotypes induced by overexpression of the SlWOX4 ortholog in tomato suggests a functional link between vascular patterning and leaf complexity.