Independent roles of the Dachshund and Eyes Absent genes in BMP signaling, axon pathfinding and neuronal specification
Independent roles of the Dachshund and Eyes Absent genes in BMP signaling, axon pathfinding and neuronal specification
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DOI：
10.1242/dev.01447
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发表时间：
2004-12-01
期刊：
影响因子：
4.6
通讯作者：
Thor, S
中科院分区：
文献类型：
--
作者：
Miguel-Aliaga, I;Allan, DW;Thor, S
在果蝇神经索中，一部分神经元表达神经肽 FMRFamide 相关 (Fmrf)。 Fmrf 表达由内在因子和外在 BMP 信号的组合代码控制。然而，这个先前确定的代码并不能完全解释 Fmrf 的规定。我们发现腊肠犬 (Dac) 和眼睛缺席 (Eya) 转录辅因子参与了这个组合密码。先前的研究已经揭示了 Dac 和 Eya 在眼睛发育过程中的密切联系。在这里，通过分析它们在具有多个表型标记的神经元中的功能，我们证明它们在神经元规范中发挥独立的作用，即使在单个细胞内也是如此。 dac 是高水平 Fmrf 表达所必需的，并且与 apterous 和 BMP 信号传导一起有效地触发 Fmrf 异位表达，甚至在运动神经元中也是如此。相比之下，eya 通过控制轴突寻路和 BMP 信号传导来调节 Fmrf 表达，但不能异位触发 Fmrf。因此，我们表明 dac 和 eya 在单一细胞类型中执行完全不同的功能，最终调节单一表型结果。
In the Drosophila nerve cord, a subset of neurons expresses the neuropeptide FMRFamide related (Fmrf). Fmrf expression is controlled by a combinatorial code of intrinsic factors and an extrinsic BMP signal. However, this previously identified code does not fully explain the regulation of Fmrf. We have found that the Dachshund (Dac) and Eyes Absent (Eya) transcription co-factors participate in this combinatorial code. Previous studies have revealed an intimate link between Dac and Eya during eye development. Here, by analyzing their function in neurons with multiple phenotypic markers, we demonstrate that they play independent roles in neuronal specification, even within single cells. dac is required for high-level Fmrf expression, and acts potently together with apterous and BMP signaling to trigger Fmrf expression ectopically, even in motoneurons. By contrast, eya regulates Fmrf expression by controlling both axon pathfinding and BMP signaling, but cannot trigger Fmrf ectopically. Thus, we show that dac and eya perform entirely different functions in a single cell type to ultimately regulate a single phenotypic outcome.