Simultaneous quantitative analysis of main components in linderae reflexae radix with one single marker.
Simultaneous quantitative analysis of main components in linderae reflexae radix with one single marker.
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DOI：
10.1080/10826076.2016.1169429
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发表时间：
2016-05-08
影响因子：
1.3
通讯作者：
Chen SQ
中科院分区：
文献类型：
--
作者：
Wang LL;Zhang YB;Sun XY;Chen SQ
建立单标记多成分定量分析（QAMS）质量评价方法，并通过同时定量测定乌药中四种主要成分验证其可行性。选择松菌素、松香素、松香素和 3,5-二羟基-2-(1-对薄荷烯基)-反式二苯乙烯的四种主要成分作为分析物，通过 RP-HPLC 结合 UV 检测器来评估质量。通过比较外标法与不同 HPLC 系统的 QAMS 的定量结果来评价该方法。结果表明，外标法与QAMS测定的乌药4种含量的定量结果无显着性差异（RSD<3%）。采用松西林单一标记法测定了乌药中4种分析物（松西林、松香素、松脂菌素和Reflexanbene I）的含量。该指纹图谱是通过配备三种不同色谱柱的 Shimadzu LC-20AT 和 Waters e2695 HPLC 测定的光谱。
Establish a quantitative analysis of multi-components by the single marker (QAMS) method for quality evaluation and validate its feasibilities by the simultaneous quantitative assay of four main components in Linderae Reflexae Radix. Four main components of pinostrobin, pinosylvin, pinocembrin, and 3,5-dihydroxy-2-(1-p-mentheneyl)-trans-stilbene were selected as analytes to evaluate the quality by RP-HPLC coupled with a UV-detector. The method was evaluated by a comparison of the quantitative results between the external standard method and QAMS with a different HPLC system. The results showed that no significant differences were found in the quantitative results of the four contents of Linderae Reflexae Radix determined by the external standard method and QAMS (RSD <3%). The contents of four analytes (pinosylvin, pinocembrin, pinostrobin, and Reflexanbene I) in Linderae Reflexae Radix were determined by the single marker of pinosylvin. This fingerprint was the spectra determined by Shimadzu LC-20AT and Waters e2695 HPLC that were equipped with three different columns.