Recent Advances in Light-Induced Cell Sheet Technology
Recent Advances in Light-Induced Cell Sheet Technology
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DOI：
10.2139/ssrn.3674117
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发表时间：
2020-09
期刊：
影响因子：
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通讯作者：
Zhiwei Jiang;Danji Zhu;Ke Yu;Yue Xi;Xiaozhao Wang;Guoli Yang
中科院分区：
文献类型：
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作者：
Zhiwei Jiang;Danji Zhu;Ke Yu;Yue Xi;Xiaozhao Wang;Guoli Yang
已应用各种刺激来收获完整的细胞片，包括温度、磁力、pH 值和电刺激。细胞片层技术是一种方便有效的方法，对组织再生和细胞治疗具有有益的效果。不同波长的光，如紫外线 (UV)、可见光和近红外线 (NIR) 光，被证实有助于制造细胞片。在光照下培养表面的润湿性、电位或含水量发生变化，引起粘附蛋白或胶原蛋白的构象变化，从而导致细胞片层脱离。然而，当前的方法面临一些限制，因为迄今为止提出的安全光照明标准很少，并且需要仔细控制波长、功率和照射时间。未来的研究应该旨在产生用于快速有效地培养和释放细胞片的新材料。
Various stimuli have been applied to harvest complete cell sheets, including temperature, magnetic, pH, and electrical stimuli. Cell sheet technology is a convenient and efficient approach with beneficial effects for tissue regeneration and cell therapy. Lights of different wavelengths, such as ultraviolet (UV), visible light, and near infrared ray (NIR) light, were confirmed to aid in fabricating a cell sheet. Changes in the wettability, potential, or water content of the culturing surfaces that occur under light illumination induce conformational changes in the adhesive proteins or collagens, which then leads to cell sheet detachment. However, the current approaches face several limitations, as few standards for safe light illumination have been proposed to date, and require a careful control of the wavelength, power, and irradiation time. Future studies should aim at generating new materials for culturing and releasing cell sheets rapidly and effectively.