Age- and gender-related trends in the expression of glutathione S-transferases in human nasal mucosa.
Age- and gender-related trends in the expression of glutathione S-transferases in human nasal mucosa.
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人鼻粘膜谷胱甘肽 S-转移酶表达的年龄和性别相关趋势。
DOI：
10.1177/000348949510401012
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Getchell,ML
中科院分区：
文献类型：
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作者：
Krishna,NS;Getchell,TV;Dhooper,N;Awasthi,YC;Getchell,ML
应用免疫细胞化学技术研究了α、μ和π型谷胱甘肽S-转移酶（GST）在人鼻粘膜细胞中的表达。在嗅粘膜中，GST-α的免疫反应在Bowman腺的腺泡细胞中最强，而在支持细胞的核上区免疫反应较弱。GST-π仅定位于支持细胞，未检测到GST-μ。在呼吸道粘膜中，GST-α和GST-π在纤毛柱状上皮细胞刷状缘处有表达。GST-α在嗅粘膜中的表达存在年龄和性别相关的趋势，而GST-π在嗅粘膜中的表达无明显差异。老年人嗅粘膜的免疫反应强度降低。GST-α在各年龄组女性嗅粘膜中的表达强度均高于男性。在呼吸道粘膜中未观察到这些差异。这些结果表明，腺泡细胞的Bowman的腺和支持细胞的第二阶段生物转化在人鼻粘膜的主要网站。
The cellular expression of α, μ, and π classes of glutathioneS-transferases (GSTs) was investigated in human nasal mucosa by means of immunocytochemical techniques. In the olfactory mucosa, immunoreactivity for GST-α was most intense in the acinar cells of the Bowman's glands, with weak immunoreactivity in the supranuclear region of sustentacular cells. Whereas GST-π was localized only in the sustentacular cells, no GST-μ was detected. In the respiratory mucosa, GST-α and GST-π were detected at the brush borders of ciliated columnar epithelial cells. There were age- and gender-related trends in the expression of GST-α, but not GST-π, in the olfactory mucosa. The intensity of immunoreactivity in the olfactory mucosa was decreased in older subjects. The expression of GST-α in the olfactory mucosa of females consistently exhibited greater intensity than that of males at all the ages studied. These differences were not observed in the respiratory mucosa. These results indicate that acinar cells of the Bowman's glands and sustentacular cells are the major sites of phase II biotransformation in the human nasal mucosa.