An epigenetic regulator emerges as microtubule minus-end binding and stabilizing factor in mitosis.
An epigenetic regulator emerges as microtubule minus-end binding and stabilizing factor in mitosis.
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DOI：
10.1038/ncomms8889
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发表时间：
2015-08-05
影响因子：
16.6
通讯作者：
Akhtar A
中科院分区：
文献类型：
--
作者：
Meunier S;Shvedunova M;Van Nguyen N;Avila L;Vernos I;Akhtar A
进化保守的NSL复合体是一个重要的表观遗传调节因子，控制着数千个基因的表达。在这里，我们揭示了NSL复合体成员在有丝分裂中的一个新功能。随着细胞进入有丝分裂，KANSL1和KANSL3经历了从染色质到有丝分裂纺锤体的显著重新定位。通过以RanGTP依赖的方式稳定微管负端，它们对于纺锤体组装和染色体分离是必不可少的。此外，我们发现KANSL3是一种微管负端结合蛋白，揭示了一类新的有丝分裂特异的微管负端调节因子。KANSL蛋白通过在间期和有丝分裂中采用不同的功能，为协调细胞周期不同阶段基因组的忠实表达和遗传任务提供了联系。七聚体KAT8相关的非特异性致死复合体由高度保守的染色质修饰蛋白组成。在这里，作者展示了复合体的成员在有丝分裂过程中调节微管组装的作用。
The evolutionary conserved NSL complex is a prominent epigenetic regulator controlling expression of thousands of genes. Here we uncover a novel function of the NSL complex members in mitosis. As the cell enters mitosis, KANSL1 and KANSL3 undergo a marked relocalisation from the chromatin to the mitotic spindle. By stabilizing microtubule minus ends in a RanGTP-dependent manner, they are essential for spindle assembly and chromosome segregation. Moreover, we identify KANSL3 as a microtubule minus-end-binding protein, revealing a new class of mitosis-specific microtubule minus-end regulators. By adopting distinct functions in interphase and mitosis, KANSL proteins provide a link to coordinate the tasks of faithful expression and inheritance of the genome during different phases of the cell cycle. The heptameric KAT8-associated nonspecific lethal complex consists of highly conserved chromatin modifier proteins. Here, the authors show a role for the members of the complex in regulating microtubule assembly during mitosis.