Regulation of the tryptophan biosynthetic genes in Bacillus halodurans: common elements but different strategies than those used by Bacillus subtilis.
Regulation of the tryptophan biosynthetic genes in Bacillus halodurans: common elements but different strategies than those used by Bacillus subtilis.
复制标题
耐盐芽孢杆菌色氨酸生物合成基因的调控：常见元件，但与枯草芽孢杆菌使用的策略不同。
DOI：
10.1128/jb.186.3.818-828.2004
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发表时间：
2004
影响因子：
3.2
通讯作者：
Gollnick,Paul
中科院分区：
文献类型：
--
作者：
Szigeti,Reka;Milescu,Mirela;Gollnick,Paul
在枯草芽孢杆菌中，一种名为TRAP的RNA结合蛋白调节色氨酸生物合成基因的转录和翻译。嗜盐芽孢杆菌是一种嗜碱性芽孢杆菌，生长在较高的pH值。以前对这种细菌的研究主要集中在碱性环境中适应生长的机制上。我们已经表征了色氨酸生物合成基因INB的调节。Haloduransand将其与INB进行了比较。枯草杆菌。嗜盐芽孢杆菌编码一个与theb有71%序列同源性的TRAP蛋白。枯草杆菌蛋白。邻氨基苯甲酸合成酶是色氨酸合成途径中的第一个酶，它的表达受到INB的调控明显较少。盐碱菌INB.枯草杆菌。对银行控制权的审查。体内和体外的卤虫trpEDCFBA操纵子都表明，只有转录受到调控，而INB则受到调控。枯草杆菌操纵子的转录和trpE的翻译都受到控制。控制转录INB的衰减机制。卤虫与INB相似。Subtilis，但在预测的RNA二级结构上存在一定的差异。Halodurans trpleader区域，包括潜在的抗终止子结构的存在。TrpG位于两种细菌的叶酸操纵子中，其翻译在两个物种中受到类似的调控。
InBacillus subtilis, an RNA binding protein called TRAP regulates both transcription and translation of the tryptophan biosynthetic genes.Bacillus haloduransis an alkaliphilicBacillusspecies that grows at high pHs. Previous studies of this bacterium have focused on mechanisms of adaptation for growth in alkaline environments. We have characterized the regulation of the tryptophan biosynthetic genes inB. haloduransand compared it to that inB. subtilis. B. haloduransencodes a TRAP protein with 71% sequence identity to theB. subtilisprotein. Expression of anthranilate synthetase, the first enzyme in the pathway to tryptophan, is regulated significantly less inB. haloduransthan inB. subtilis. Examination of the control of theB. halodurans trpEDCFBAoperon both in vivo and in vitro shows that only transcription is regulated, whereas inB. subtilisboth transcription of the operon and translation oftrpEare controlled. The attenuation mechanism that controls transcription inB. haloduransis similar to that inB. subtilis, but there are some differences in the predicted RNA secondary structures in theB. halodurans trpleader region, including the presence of a potential anti-antiterminator structure. Translation oftrpG, which is within the folate operon in both bacilli, is regulated similarly in the two species.