Expression of secretory proteins in oral fluid after orthodontic tooth movement
Expression of secretory proteins in oral fluid after orthodontic tooth movement
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DOI：
10.1067/mod.2002.121011
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发表时间：
2002-03-01
影响因子：
3
通讯作者：
Mednieks, MI
中科院分区：
文献类型：
--
作者：
Burke, JC;Evans, CA;Mednieks, MI
正畸治疗改变了骨和口腔局部分泌蛋白的表达，但其全身效应尚不清楚。总分泌蛋白质和环磷酸腺苷（AMP）依赖性蛋白激酶亚基（RII）测定唾液和龈沟液（GCF）后，正畸分离器的位置，以确定是否施加到牙齿的机械力影响蛋白质分泌。在治疗前和治疗后1天收集全唾液和GCF。进行电泳和Western印迹以建立总蛋白的带型，并测量作为表观应激指标的RII的同种型和量。数字化图像文件用于RII和总蛋白的相对浓度的光密度分析。个体蛋白值显示唾液或GCF无统计学显著变化。然而，Western印迹显示，在放置分离器后，RII出现了显著的差异：50-55 kd的条带几乎消失，取而代之的是20 kd范围内的片段。这些结果表明，虽然总蛋白质的表达不改变机械力施加到牙齿，通过环AMP信号通路的全身反应可能已被激活。
Orthodontic treatment alters the expression of secretory proteins at the local level in bone and the oral cavity, but its systemic effects are not well understood. Total secretory proteins and a cyclic adenosine monophosphate (AMP)-dependent protein kinase subunit (RII) were measured in saliva and gingival crevicular fluid (GCF) after the placement of orthodontic separators to determine if mechanical force applied to teeth affects protein secretion. Whole saliva and GCF were collected before and 1 day after treatment. Electrophoresis and Western blotting were carried out to establish the banding patterns of total proteins and to measure the isotype and amount of RII that serves as an apparent stress indicator. Digitized image files were used for densitometric analyses of the relative concentrations of RII and total protein. Individual protein values showed no statistically significant changes in saliva or GCF. Western blots, however, showed a dramatic difference in RII after the placement of separators: the 50-to-55 kilodaltons (kd) band virtually disappeared and was replaced by a fragment in the 20-kd range. These results suggest that although the expression of total proteins is not altered by mechanical force applied to teeth, a systemic response via the cyclic AMP signaling pathway might have been activated.