Structure of rice-straw arabinoglucuronoxylan and specificity of Streptomyces xylanase toward the xylan.
Structure of rice-straw arabinoglucuronoxylan and specificity of Streptomyces xylanase toward the xylan.
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稻秆阿拉伯葡糖醛酸木聚糖的结构和链霉菌木聚糖酶对木聚糖的特异性。
DOI：
10.1080/00021369.1990.10869948
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
K. Murakami
中科院分区：
文献类型：
--
作者：
S. Yoshida;I. Kusakabe;N. Matsuo;K. Shimizu;T. Yasui;K. Murakami
用5%氨水处理的稻草被链霉菌属的β-木聚糖酶水解。通过活性炭柱、凝胶过滤、离子交换和氨丙基硅胶柱色谱从水解产物中分离出E-86和四种杂寡糖，两种阿拉伯木寡糖和两种葡萄糖醛酸木寡糖。通过成分糖分析、部分酸水解、基于上述寡糖的结构，讨论了稻草阿拉伯葡糖醛酸木聚糖的结构以及木聚糖酶对木聚糖的特异性。
Rice-straw treated with 5% ammonia water was hydrolyzed with the beta-xylanase of Streptomyces sp. E-86, and four kinds of hetero-oligosaccharides, two arabinoxylo- and two glucuronoxylo-oligosaccharides, were isolated from the hydrolysate by charcoal column, gel filtration, ion-exchange, and aminopropyl silica column chromatographies. The structures of the oligosaccharides were identified as 3(2)-alpha-L-arabinofuranosyl-xylobiose, 3(2)-alpha-L-arabinofuranosylxylotriose, 2(3)-4-O-methyl-alpha-D-glucuronosylxylotriose, and 2(3)-alpha-D-glucuronosylxylotriose by the analysis of component sugar, partial acid hydrolysis, methylation analysis etc. Based on the structures of above oligosaccharides, the structure of rice-straw arabinoglucuronoxylan and the specificity of the xylanase toward the xylan are discussed.