Signal and measurement considerations for human translation of diffuse in vivo flow cytometry.
Signal and measurement considerations for human translation of diffuse in vivo flow cytometry.
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DOI：
10.1117/1.jbo.27.6.067001
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发表时间：
2022-06
影响因子：
3.5
通讯作者：
Niedre, Mark
中科院分区：
文献类型：
--
作者：
Ivich, Fernando;Pace, Joshua;Williams, Amber L.;Shumel, Malcolm;Fang, Qianqian;Niedre, Mark
“弥散体内流式细胞术”（DiFC）是一种新兴的技术，用于直接在小鼠的大型深层血管中荧光检测稀有循环细胞。由于DiFC使用高度散射的光，原则上，它可以转化为人类使用。然而，一个悬而未决的问题是，来自单细胞的荧光信号是否可以在人类规模的解剖结构中检测到。人手腕或前臂中合适的血管在4mm的深度处。本工作的目的是研究DiFC仪器的几何形状和波长对检测到的DiFC信号和对移动细胞的最大检测深度的影响。我们使用Monte Carlo模拟来计算荧光雅可比（灵敏度）矩阵的范围内的源和检测器分离（SDS）和组织的光学特性在可见光和近红外光谱。我们进行了实验测量与三个可用版本的DiFC（488，640和780 nm），荧光微球，和组织模仿光流体。我们使用计算和实验数据来估计在每个设置组合下的最大检测深度。对于DiFC检测问题，我们的分析表明，对于深层血管，使用NIR光（780 nm）和3 mm SDS获得最大灵敏度。这些结果表明，结合合适的分子靶向荧光探针，循环细胞和纳米传感器原则上可以在人体循环中检测到。
“Diffuse in vivo flow cytometry” (DiFC) is an emerging technology for fluorescence detection of rare circulating cells directly in large deep-seated blood vessels in mice. Because DiFC uses highly scattered light, in principle, it could be translated to human use. However, an open question is whether fluorescent signals from single cells would be detectable in human-scale anatomies. Suitable blood vessels in a human wrist or forearm are at a depth of to 4 mm. The aim of this work was to study the impact of DiFC instrument geometry and wavelength on the detected DiFC signal and on the maximum depth of detection of a moving cell. We used Monte Carlo simulations to compute fluorescence Jacobian (sensitivity) matrices for a range of source and detector separations (SDS) and tissue optical properties over the visible and near infrared spectrum. We performed experimental measurements with three available versions of DiFC (488, 640, and 780 nm), fluorescent microspheres, and tissue mimicking optical flow phantoms. We used both computational and experimental data to estimate the maximum depth of detection at each combination of settings. For the DiFC detection problem, our analysis showed that for deep-seated blood vessels, the maximum sensitivity was obtained with NIR light (780 nm) and 3-mm SDS. These results suggest that—in combination with a suitable molecularly targeted fluorescent probes—circulating cells and nanosensors could, in principle, be detectable in circulation in humans.
影响因子：
3.5
作者：
Zettergren E;Swamy T;Runnels J;Lin CP;Niedre M
通讯作者：
Niedre M