Crystallization of the B chain of Shiga-like toxin I from Escherichia coli.
Crystallization of the B chain of Shiga-like toxin I from Escherichia coli.
复制标题
来自大肠杆菌的志贺样毒素 I 的 B 链结晶。
DOI：
10.1016/0022-2836(91)90256-6
复制
发表时间：
1991
影响因子：
5.6
通讯作者：
Robertus,JD
中科院分区：
文献类型：
--
作者：
Hart,PJ;Monzingo,AF;Donohue-Rolfe,A;Keusch,GT;Calderwood,SB;Robertus,JD
Shiga-like toxin I (SLT-I) is produced by several pathogenic strains of Escherichia coli associated with diarrheal disease. The toxin consists of an A chain, which attacks eukaryotic ribosomes, inhibiting protein synthesis, and multiple copies of a 69 ammo acid B chain. The B subunit mediates cell binding and uptake through its interactions with cell surface carbohydrate moieties. Here we report that the B chain has been crystallized in a form suitable for high-resolution X-ray analysis. The space group is P2 1 2 1 2 1, with a= 56.2 A ̊, b= 59.9 A ̊ and c= 102.5 A ̊. A rotation function using three-dimensional diffraction data suggests that the asymmetric unit is a tetramer.
