BINDING OF BETA-LACTAM ANTIBIOTICS TO PENICILLIN-BINDING PROTEINS IN METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS
BINDING OF BETA-LACTAM ANTIBIOTICS TO PENICILLIN-BINDING PROTEINS IN METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS
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DOI：
10.1093/infdis/161.6.1170
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发表时间：
1990-06-01
影响因子：
6.4
通讯作者：
SACHDEVA, M
中科院分区：
文献类型：
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作者：
CHAMBERS, HF;SACHDEVA, M
金黄色葡萄球菌的青霉素结合蛋白（PBP）对几种β-青霉素的结合亲和力在甲氧西林敏感菌株和两种甲氧西林耐药菌株（一种是异质的，一种是同质的）中检测内酰胺抗生素，以确定PBP结合和耐药性表达之间的关系。PBPs 1-4在所有三种菌株中具有相似的亲和力。PBP 2a亲和力在两种耐药菌株中相似。对于敏感菌株和异质菌株中的敏感细胞亚群，生长抑制与PBPs 1-3的结合相关，表明这些PBPs介导两者的易感性。对于耐药细胞，生长抑制仅与结合PBP 2a相关，表明这是耐药细胞中的靶点。对PBP 2a的结合亲和力或抑制最具抗性的细胞亚群的生长的浓度的测定应包括在β-CD的评价中。内酰胺类抗生素对耐甲氧西林的S.金黄色。
Binding affinity of penicillin-binding proteins (PBP) of Staphylococcus aureus for several .beta.-lactam antibiotics was examined in a methicillin-susceptible strain and in two methicillin-resistant strains, one heterogeneous and one homogenous, to determine relationships between PBP binding and expression of resistance. PBPs 1-4 had similar affinities in all three strains. PBP 2a affinities were similar in both resistant strains. For the susceptible strain and for the susceptible subpopulation of cells in the heterogeneous strain, growth inhibition correlated with binding to PBPs 1-3, suggesting that these PBPs mediate susceptibility in both. For resistant cells, growth inhibition correlated only with binding to PBP 2a, suggesting that this is the target in resistant cells. Determination of binding affinity for PBP 2a or the concentration that inhibits growth of the most resistant subpopulation of cells should be included in evaluation of .beta.-lactam antibiotics for potential activity against methicillin-resistant strains of S. aureus.