Axonal swellings predict the degeneration of epidermal nerve fibers in painful neuropathies
Axonal swellings predict the degeneration of epidermal nerve fibers in painful neuropathies
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DOI：
10.1212/01.wnl.0000070781.92512.a4
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发表时间：
2003-09-09
期刊：
影响因子：
9.9
通讯作者：
Pareyson, D
中科院分区：
文献类型：
--
作者：
Lauria, G;Morbin, M;Pareyson, D
目的：将表皮内神经纤维（IENF）的密度与疼痛性神经病患者表皮神经支配密度的纵向测量相关联，并评估IENF肿胀对神经病进展的预测价值。研究方法：对15例持续性足部疼痛的患者进行了神经学检查、神经传导研究、定量感觉检查和大腿近端和小腿远端皮肤活检。在所有患者和15名健康受试者中，在下肢远端定量IENF密度和肿胀比（sleep/IENF）。平均19.2个月后进行随访研究，包括IENF密度和肿胀率定量。使用抗人蛋白质基因产物9.5、抗微管、抗神经丝和抗突触素抗体进行双染色共聚焦显微镜研究，以评估sleep内的特异性蓄积。并对IENF进行了超微结构观察。结果：神经病变组IENF密度低于正常对照组，肿胀率高于正常对照组（p
Objective: To correlate the density of swellings in intraepidermal nerve fibers (IENF) with the longitudinal measurement of the epidermal innervation density in patients with painful neuropathy and to assess the predictive value of IENF swelling to progression of neuropathy. Methods: Fifteen patients with persistent pain in the feet underwent neurologic examination, nerve conduction studies, quantitative sensory examination, and skin biopsies at proximal thigh and distal leg. In all patients and in 15 healthy subjects, IENF density and swelling ratio (no. swellings/no. IENF) were quantified at distal leg. Follow-up study, including IENF density and swelling ratio quantification, was performed a mean of 19.2 months later. Double staining confocal microscope studies using anti-human protein-gene-product 9.5, anti-tubule, anti-neurofilament, and anti-synaptophysin antibodies were performed to assess specific accumulation within swellings. Ultrastructural investigation of IENF was also carried out. Results: Patients with neuropathy had lower density of IENF and higher swelling ratio than healthy subjects (p