Subliminal light control of dark adaptation kinetics in Phycomyces phototropism.
Subliminal light control of dark adaptation kinetics in Phycomyces phototropism.
复制标题
须霉菌向光性暗适应动力学的阈下光控制。
DOI：
10.1111/j.1751-1097.1989.tb09199.x
复制
发表时间：
1989
影响因子：
3.3
通讯作者：
Lipson,ED
中科院分区：
文献类型：
--
作者：
Galland,P;Corrochano,LM;Lipson,ED
植藻向光性的暗适应动力学主要取决于实验方案。当在447 nm照射速率为1 W m2的光适应孢子体暴露在微弱的单侧光下时，适应动力学呈指数衰减（6分钟时间常数）。然而，当适应光的孢子囊体在黑暗中（即在传统的红色安全灯下）保持不同的时间间隔，然后暴露在昏暗的单侧测试光下时，适应的衰减动力学呈双指数，在第一分钟（1分钟时间常数）快速衰减，随后缓慢恢复（11分钟时间常数）。因此，在孢子囊适应1 W m - 2后，给予的微弱的潜意识光实际上是被感知的，并对黑暗适应过程施加了控制。观察到的暗适应动力学的加速构成了孢子囊的一种新的光效应。在波长383 nm处，没有观察到这种效应。由于缺乏β -胡萝卜素的突变体L91（基因型carb）在存在或不存在阈下光的情况下，在暗适应动力学中没有改变，因此β -胡萝卜素似乎不参与适应的光控制。
The dark adaptation kinetics ofPhycomycesphototropism depend critically on the experimental protocol. When sporangiophores that had been light‐adapted to a fluence rate of 1 W m2at 447 nm were exposed to dim unilateral light, the adaptation kinetics showed exponential decay (6 min time constant). However, when light‐adapted sporangiophores were kept for variable intervals in darkness (i.e. in presence of traditional red safelight) and then exposed to dim unilateral test light, the decay kinetics of adaptation were biexponential with a rapid decay during the first minute (1 min time constant), followed by a slow recovery (11 min time constant). Thus, the dim subliminal light given after the sporangiophores had been adapted to 1 W m‐2, was actually perceived, and exerted control over the dark‐adaptation process. The observed acceleration of dark‐adaptation kinetics constitutes a novel light effect of the sporangiophore. At wavelength 383 nm this effect was not observed. Because a β‐carotene lacking mutant, L91 (genotypecarB), was unmodified in dark‐adaptation kinetics measured in the presence or absence of subliminal light, it appears that β‐carotene is not involved in the photocontrol of adaptation.